2-\u6eb4-7-(\u53d4\u4e01\u6c27\u57fa\u7fb0\u57fa)-5,6,7,8-\u56db\u6c22\u54aa\u5511\u5e76[1,2-A]\u5421\u55ea-3-\u7fa7\u9178\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u672c\u4ea7\u54c1\u4e3a\u9ad8\u7eaf\u5ea6\u6709\u673a\u5316\u5408\u7269\uff0c\u5316\u5b66\u540d\u79f0\u4e3a2-Bromo-7-(tert-butoxycarbonyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyrazine-3-carboxylic acid\uff0cCAS\u53f7\u4e3a1823840-73-8\u3002\u5176\u5206\u5b50\u5f0f\u4e3aC12H16BrN3O4\uff0c\u5206\u5b50\u91cf\u4e3a346.18\uff0c\u5e38\u6e29\u4e0b\u5448\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7ed3\u6676\u7c89\u672b\u3002\u8be5\u5316\u5408\u7269\u542b\u6709\u6eb4\u539f\u5b50\u3001\u53d4\u4e01\u6c27\u7fb0\u57fa\uff08Boc\uff09\u4fdd\u62a4\u57fa\u56e2\u4ee5\u53ca\u7fa7\u9178\u5b98\u80fd\u56e2\uff0c\u7ed3\u6784\u4e2d\u7684\u54aa\u5511\u5e76\u5421\u55ea\u9aa8\u67b6\u8d4b\u4e88\u5176\u72ec\u7279\u7684\u5316\u5b66\u6027\u8d28\uff0c\u4f7f\u5176\u5728\u6709\u673a\u5408\u6210\u548c\u836f\u7269\u5316\u5b66\u4e2d\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u8be5\u5316\u5408\u7269\u662f\u4e00\u79cd\u91cd\u8981\u7684\u533b\u836f\u4e2d\u95f4\u4f53\uff0c\u5176\u7ed3\u6784\u4e2d\u7684Boc\u4fdd\u62a4\u57fa\u56e2\u53ef\u9009\u62e9\u6027\u8131\u9664\uff0c\u4fbf\u4e8e\u540e\u7eed\u5b98\u80fd\u56e2\u4fee\u9970\u3002\u6eb4\u539f\u5b50\u7684\u5b58\u5728\u4f7f\u5176\u6613\u4e8e\u53c2\u4e0e\u5076\u8054\u53cd\u5e94\uff08\u5982Suzuki\u5076\u8054\uff09\uff0c\u800c\u7fa7\u9178\u57fa\u56e2\u53ef\u8fdb\u4e00\u6b65\u884d\u751f\u4e3a\u9170\u80fa\u6216\u916f\u7c7b\u5316\u5408\u7269\u3002\u8fd9\u4e9b\u7279\u6027\u4f7f\u5176\u5728\u6784\u5efa\u590d\u6742\u6742\u73af\u4f53\u7cfb\u3001\u5f00\u53d1\u86cb\u767d\u9176\u6291\u5236\u5242\u6216\u6fc0\u9176\u9776\u5411\u836f\u7269\u4e2d\u5177\u6709\u5e7f\u6cdb\u5e94\u7528\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u672c\u4ea7\u54c1\u4e3b\u8981\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a
\n3.1 \u836f\u7269\u7814\u53d1\uff1a\u4f5c\u4e3a\u5173\u952e\u4e2d\u95f4\u4f53\u7528\u4e8e\u5408\u6210\u6297\u80bf\u7624\u3001\u6297\u75c5\u6bd2\u6216\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u836f\u7269\u3002
\n3.2 \u6709\u673a\u5408\u6210\uff1a\u7528\u4e8e\u6784\u5efa\u542b\u6c2e\u6742\u73af\u5316\u5408\u7269\uff0c\u6216\u4f5c\u4e3a\u624b\u6027\u5408\u6210\u5b50\u53c2\u4e0e\u4e0d\u5bf9\u79f0\u50ac\u5316\u53cd\u5e94\u3002
\n3.3 \u751f\u5316\u63a2\u9488\u5f00\u53d1\uff1a\u901a\u8fc7\u884d\u751f\u5316\u6807\u8bb0\u751f\u7269\u5927\u5206\u5b50\uff0c\u7528\u4e8e\u4f5c\u7528\u673a\u5236\u7814\u7a76\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n4.1 \u50a8\u5b58\u6761\u4ef6\uff1a\u5efa\u8bae\u5bc6\u5c01\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u907f\u514d\u5149\u7167\u548c\u6e7f\u6c14\u3002\u957f\u671f\u50a8\u5b58\u9700\u5145\u5165\u60f0\u6027\u6c14\u4f53\u4fdd\u62a4\u3002
\n4.2 \u4f7f\u7528\u5efa\u8bae\uff1a\u79f0\u53d6\u65f6\u9700\u5728\u5e72\u71e5\u73af\u5883\u4e0b\u64cd\u4f5c\uff0c\u5efa\u8bae\u4f7f\u7528\u6c2e\u6c14\u4fdd\u62a4\u3002\u6eb6\u89e3\u6027\u6d4b\u8bd5\u8868\u660e\uff0c\u8be5\u5316\u5408\u7269\u6613\u6eb6\u4e8e\u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u3001\u4e8c\u6c2f\u7532\u70f7\u7b49\u6709\u673a\u6eb6\u5242\uff0c\u6c34\u6eb6\u6027\u8f83\u5dee\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n5.1 \u8d28\u91cf\u63a7\u5236\uff1a\u901a\u8fc7HPLC\u68c0\u6d4b\u7eaf\u5ea6\u226596%\uff0c\u6279\u6b21\u95f4\u63d0\u4f9bCOA\uff08\u8d28\u91cf\u5206\u6790\u8bc1\u4e66\uff09\u3002
\n5.2 \u5b89\u5168\u4fe1\u606f\uff1a\u672c\u54c1\u5bf9\u773c\u775b\u548c\u76ae\u80a4\u6709\u523a\u6fc0\u6027\uff0c\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u624b\u5957\u53ca\u62a4\u76ee\u955c\u3002\u82e5\u63a5\u89e6\u76ae\u80a4\uff0c\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u7167\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002 <\/p>\n
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