4-((Biotinoyl)amino)Butyric acid\uff08\u751f\u7269\u7d20\u9170\u57fa-4-\u6c28\u57fa\u4e01\u9178\uff09\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u672c\u4ea7\u54c1\u4e3a\u751f\u7269\u7d20\u884d\u751f\u7269\uff0c\u5316\u5b66\u540d\u79f0\u4e3a4-((Biotinoyl)amino)Butyric acid\uff0cCAS\u53f735924-87-9\uff0c\u5206\u5b50\u5f0fC15H23N3O6S\uff0c\u5206\u5b50\u91cf373.42462\u3002\u5176\u7ed3\u6784\u7531\u751f\u7269\u7d20\uff08\u7ef4\u751f\u7d20H\uff09\u901a\u8fc7\u9170\u80fa\u952e\u4e0e4-\u6c28\u57fa\u4e01\u9178\u8fde\u63a5\u800c\u6210\uff0c\u7eaf\u5ea6>96%\uff0c\u5448\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7c89\u672b\u72b6\u3002\u8be5\u5316\u5408\u7269\u517c\u5177\u751f\u7269\u7d20\u7684\u9ad8\u4eb2\u548c\u529b\u7279\u6027\u4e0e\u7fa7\u57fa\u7684\u53cd\u5e94\u6d3b\u6027\uff0c\u53ef\u6eb6\u4e8eDMSO\u3001\u7532\u9187\u7b49\u6709\u673a\u6eb6\u5242\uff0c\u5728\u6c34\u6eb6\u6db2\u4e2d\u6eb6\u89e3\u5ea6\u8f83\u4f4e\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4f5c\u4e3a\u751f\u7269\u7d20\u5316\u8bd5\u5242\uff0c\u672c\u4ea7\u54c1\u53ef\u901a\u8fc7\u7fa7\u57fa\u4e0e\u6c28\u57fa\u7684\u7f29\u5408\u53cd\u5e94\uff08\u5982EDC\/NHS\u6d3b\u5316\uff09\u5c06\u751f\u7269\u7d20\u6807\u8bb0\u81f3\u86cb\u767d\u8d28\u3001\u6838\u9178\u6216\u5176\u4ed6\u542b\u6c28\u57fa\u5206\u5b50\u3002\u751f\u7269\u7d20\u4e0e\u94fe\u9709\u4eb2\u548c\u7d20\/\u4eb2\u548c\u7d20\u7684\u5f3a\u7279\u5f02\u6027\u7ed3\u5408\uff08Kd\u224810^-15 M\uff09\u4f7f\u5176\u6210\u4e3a\u68c0\u6d4b\u3001\u7eaf\u5316\u7cfb\u7edf\u7684\u6838\u5fc3\u5de5\u5177\u3002\u51764-\u78b3\u95f4\u9694\u81c2\u53ef\u51cf\u5c11\u7a7a\u95f4\u4f4d\u963b\uff0c\u663e\u8457\u63d0\u9ad8\u6807\u8bb0\u6548\u7387\uff0c\u9002\u7528\u4e8e\u9700\u8981\u9ad8\u7075\u654f\u5ea6\u68c0\u6d4b\u7684\u5206\u5b50\u4e92\u4f5c\u7814\u7a76\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u8be5\u4ea7\u54c1\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5076\u8054\u9886\u57df\uff1a
\n– \u86cb\u767d\u8d28\u6807\u8bb0\uff1a\u7528\u4e8eWestern blot\u3001ELISA\u3001\u514d\u75ab\u8367\u5149\u7b49\u68c0\u6d4b\u6280\u672f\u7684\u6297\u4f53\u751f\u7269\u7d20\u5316
\n– \u6838\u9178\u63a2\u9488\u4fee\u9970\uff1a\u5236\u5907\u751f\u7269\u7d20\u6807\u8bb0\u7684DNA\/RNA\u63a2\u9488\uff0c\u7528\u4e8e\u6742\u4ea4\u68c0\u6d4b
\n– \u7ec6\u80de\u8868\u9762\u6807\u8bb0\uff1a\u901a\u8fc7\u6c28\u57fa\u53cd\u5e94\u6807\u8bb0\u7ec6\u80de\u819c\u86cb\u767d\uff0c\u7528\u4e8e\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790
\n– \u4eb2\u548c\u5c42\u6790\uff1a\u4f5c\u4e3a\u914d\u4f53\u56fa\u5b9a\u5316\u4ecb\u8d28\uff0c\u7eaf\u5316\u751f\u7269\u7d20\u7ed3\u5408\u86cb\u767d <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u5efa\u8bae\u907f\u5149\u5bc6\u5c01\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u6709\u6548\u671f24\u4e2a\u6708\u3002\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\u5e76\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5de5\u4f5c\u6db2\u5efa\u8bae\u73b0\u914d\u73b0\u7528\uff0c\u6eb6\u5242\u9009\u62e9\u9700\u6839\u636e\u76ee\u6807\u5206\u5b50\u7279\u6027\uff08\u5982DMSO\u9002\u7528\u4e8e\u758f\u6c34\u6027\u4f53\u7cfb\uff0cPBS\u7f13\u51b2\u6db2\u9700\u914d\u5408\u6d3b\u5316\u5242\u4f7f\u7528\uff09\u3002\u6807\u8bb0\u53cd\u5e94\u9700\u63a7\u5236pH 7.0-9.0\uff0c\u63a8\u8350\u6469\u5c14\u6295\u6599\u6bd4\uff08\u8bd5\u5242:\u76ee\u6807\u5206\u5b50\uff09\u4e3a5:1\u81f320:1\u4f18\u5316\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6>96%\uff0cMS\u53caNMR\u786e\u8ba4\u7ed3\u6784\u3002\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u88c5\u5907\uff0c\u907f\u514d\u5438\u5165\u6216\u76ae\u80a4\u63a5\u89e6\u3002\u5316\u5b66\u6027\u8d28\u7a33\u5b9a\u4f46\u9700\u8fdc\u79bb\u5f3a\u6c27\u5316\u5242\uff0c\u5e9f\u5f03\u7269\u5e94\u4f5c\u4e3a\u6709\u5bb3\u5316\u5b66\u54c1\u5904\u7406\u3002\u6025\u6027\u6bd2\u6027\u6570\u636e\uff08LD50\u5927\u9f20\u53e3\u670d\uff09>2000 mg\/kg\uff0c\u82e5\u63a5\u89e6\u773c\u775b\u9700\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u5e76\u5c31\u533b\u3002 <\/p>\n
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