1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
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2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
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3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u5728\u836f\u7269\u7814\u53d1\u4e2d\uff0c\u672c\u54c1\u53ef\u7528\u4e8e\u6784\u5efa\u6297\u4f53-\u836f\u7269\u5076\u8054\u7269\uff08ADC\uff09\u6216\u5c0f\u5206\u5b50\u524d\u836f\uff1b\u5728\u5206\u5b50\u751f\u7269\u5b66\u4e2d\uff0c\u4f5c\u4e3a\u6838\u9178\u4ea4\u8054\u5242\u6216\u86cb\u767d\u8d28\u4fee\u9970\u8bd5\u5242\uff1b\u5728\u6750\u6599\u79d1\u5b66\u4e2d\uff0c\u7528\u4e8e\u529f\u80fd\u5316\u7eb3\u7c73\u9897\u7c92\u8868\u9762\u3002\u5177\u4f53\u5b9e\u9a8c\u5305\u62ec\uff1a\u5171\u4ef7\u6807\u8bb0\u751f\u7269\u5206\u5b50\u3001\u5f00\u53d1\u8bca\u65ad\u8bd5\u5242\u76d2\u3001\u8bbe\u8ba1\u81ea\u7ec4\u88c5\u8d85\u5206\u5b50\u7cfb\u7edf\u7b49\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u5efa\u8bae\u907f\u5149\u5bc6\u5c01\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u5f00\u5c01\u540e\u9700\u5145\u6c2e\u4fdd\u62a4\u3002\u4f7f\u7528\u65f6\u4ee5\u65e0\u83ccPBS\u6216DMF\u6eb6\u89e3\uff08\u63a8\u8350\u6d53\u5ea61-10 mM\uff09\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5de5\u4f5c\u6db2\u9700\u73b0\u914d\u73b0\u7528\uff0c\u957f\u671f\u5b58\u653e\u53ef\u80fd\u5bfc\u81f4\u6c34\u89e3\u3002\u4e0e\u6c28\u57fa\u53cd\u5e94\u8bd5\u5242\uff08\u5982NHS\u916f\uff09\u8054\u7528\u65f6\uff0c\u5efa\u8baepH 7-8\u7f13\u51b2\u4f53\u7cfb\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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