\u4ea7\u54c1\u8bf4\u660e\u4e66\uff1a\u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488DiR <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u7ec6\u80de\u819c\u8367\u5149\u63a2\u9488DiR\u662f\u4e00\u79cd\u8fd1\u7ea2\u5916\u8367\u5149\u67d3\u6599\uff0c\u5316\u5b66\u540d\u79f0\u4e3a2-[(1E,3E,5E,7E)-7-(3,3-Dimethyl-1-octadecyl-1,3-dihydro-2H-indol-2-ylidene)-1,3,5-heptatrien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indolium iodide\uff0cCAS\u53f7\u4e3a100068-60-8\u3002\u5176\u5206\u5b50\u5f0f\u4e3aC63H101IN2\uff0c\u5206\u5b50\u91cf\u4e3a1013.394\uff0c\u7eaf\u5ea6\u9ad8\u4e8e96%\u3002\u8be5\u5316\u5408\u7269\u5c5e\u4e8e\u83c1\u67d3\u6599\u5bb6\u65cf\uff0c\u5177\u6709\u758f\u6c34\u6027\u957f\u78b3\u94fe\u7ed3\u6784\uff0c\u53ef\u9ad8\u6548\u5d4c\u5165\u7ec6\u80de\u819c\u8102\u8d28\u53cc\u5c42\uff0c\u53d1\u5c04\u6ce2\u957f\u4f4d\u4e8e\u8fd1\u7ea2\u5916\u533a\uff08\u7ea6750 nm\uff09\uff0c\u663e\u8457\u964d\u4f4e\u751f\u7269\u7ec4\u7ec7\u81ea\u53d1\u8367\u5149\u7684\u5e72\u6270\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\nDiR\u901a\u8fc7\u5176\u4e24\u4eb2\u6027\u7ed3\u6784\u7279\u5f02\u6027\u6807\u8bb0\u7ec6\u80de\u819c\uff0c\u5728\u751f\u7406\u6761\u4ef6\u4e0b\u5448\u73b0\u6781\u4f4e\u6bd2\u6027\uff0c\u4e14\u8367\u5149\u4fe1\u53f7\u7a33\u5b9a\u3002\u5176\u8fd1\u7ea2\u5916\u53d1\u5c04\u7279\u6027\u4f7f\u5176\u5177\u5907\u6df1\u5c42\u7ec4\u7ec7\u7a7f\u900f\u80fd\u529b\uff0c\u9002\u7528\u4e8e\u6d3b\u4f53\u6210\u50cf\u7814\u7a76\u3002\u6b64\u5916\uff0cDiR\u7684\u8367\u5149\u5f3a\u5ea6\u4e0e\u819c\u7535\u4f4d\u65e0\u5173\uff0c\u53ef\u4f5c\u4e3a\u7ec6\u80de\u8fc1\u79fb\u3001\u80bf\u7624\u8f6c\u79fb\u53ca\u8840\u7ba1\u751f\u6210\u7684\u957f\u671f\u8ffd\u8e2a\u5de5\u5177\uff0c\u5728\u52a8\u6001\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u63d0\u4f9b\u9ad8\u4fe1\u566a\u6bd4\u68c0\u6d4b\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\nDiR\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u751f\u7269\u5b66\u548c\u533b\u5b66\u7814\u7a76\u9886\u57df\uff0c\u5305\u62ec\u4f46\u4e0d\u9650\u4e8e\u4ee5\u4e0b\u65b9\u5411\uff1a
\n1. \u7ec6\u80de\u819c\u6807\u8bb0\u4e0e\u8ffd\u8e2a\uff1a\u7528\u4e8e\u4f53\u5916\u6216\u4f53\u5185\u7ec6\u80de\u793a\u8e2a\uff0c\u5982\u514d\u75ab\u7ec6\u80de\u3001\u80bf\u7624\u7ec6\u80de\u7684\u8fc1\u79fb\u7814\u7a76\u3002
\n2. \u6d3b\u4f53\u6210\u50cf\uff1a\u9002\u7528\u4e8e\u5c0f\u52a8\u7269\u6a21\u578b\u4e2d\u7684\u6dcb\u5df4\u7cfb\u7edf\u3001\u8840\u7ba1\u7f51\u7edc\u53ca\u80bf\u7624\u75c5\u7076\u7684\u65e0\u521b\u53ef\u89c6\u5316\u3002
\n3. \u8102\u8d28\u4f53\u4e0e\u7eb3\u7c73\u9897\u7c92\u6807\u8bb0\uff1a\u4f5c\u4e3a\u8367\u5149\u63a2\u9488\u4fee\u9970\u836f\u7269\u8f7d\u4f53\uff0c\u76d1\u6d4b\u5176\u5728\u751f\u7269\u4f53\u5185\u7684\u5206\u5e03\u4e0e\u4ee3\u8c22\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u4ee5DMSO\u6216\u4e59\u9187\u914d\u5236\u62101-10 mM\u50a8\u5b58\u6db2\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5de5\u4f5c\u6d53\u5ea6\u901a\u5e38\u4e3a1-10 \u03bcM\uff0c\u5177\u4f53\u9700\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\u3002\u4f7f\u7528\u65f6\u9700\u6ce8\u610f\u907f\u5149\u64cd\u4f5c\uff0c\u9632\u6b62\u67d3\u6599\u6dec\u706d\uff1b\u6807\u8bb0\u7ec6\u80de\u540e\u5efa\u8bae\u6d17\u6da4\u53bb\u9664\u672a\u7ed3\u5408\u67d3\u6599\u4ee5\u964d\u4f4e\u80cc\u666f\u4fe1\u53f7\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6>96%\uff0c\u6279\u53f7\u76f8\u5173\u8d28\u68c0\u62a5\u544a\u53ef\u968f\u8d27\u63d0\u4f9b\u3002\u5b89\u5168\u64cd\u4f5c\u9700\u4f69\u6234\u9632\u62a4\u624b\u5957\u53ca\u62a4\u76ee\u955c\uff0c\u907f\u514d\u5438\u5165\u6216\u63a5\u89e6\u76ae\u80a4\u3002\u5982\u4e0d\u614e\u63a5\u89e6\uff0c\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u5e76\u5c31\u533b\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002 <\/p>\n
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