2′-\u8131\u6c27\u5c3f\u82f7-5′-\u4e09\u78f7\u9178\u4e09\u94a0\u76d0\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n2′-\u8131\u6c27\u5c3f\u82f7-5′-\u4e09\u78f7\u9178\u4e09\u94a0\u76d0\uff082′-Deoxyuridine-5′-triphosphate disodium salt\uff09\u662f\u4e00\u79cd\u91cd\u8981\u7684\u6838\u82f7\u9178\u884d\u751f\u7269\uff0c\u5316\u5b66\u5f0f\u4e3aC9H13N2Na2O14P3\uff0c\u5206\u5b50\u91cf512.105\uff0cCAS\u53f7102814-08-4\u3002\u672c\u54c1\u4ee5\u4e09\u94a0\u76d0\u5f62\u5f0f\u5b58\u5728\uff0c\u7eaf\u5ea6\u8d85\u8fc796%\uff0c\u4e3a\u767d\u8272\u6216\u7c7b\u767d\u8272\u51bb\u5e72\u7c89\u672b\uff0c\u6613\u6eb6\u4e8e\u6c34\uff0c\u5728\u751f\u7406pH\u6761\u4ef6\u4e0b\u7a33\u5b9a\u3002\u5176\u7ed3\u6784\u5305\u542b\u8131\u6c27\u5c3f\u82f7\u78b1\u57fa\u3001\u4e09\u78f7\u9178\u57fa\u56e2\u53ca\u94a0\u79bb\u5b50\uff0c\u662fDNA\u5408\u6210\u4e0e\u4fee\u590d\u7684\u5173\u952e\u5e95\u7269\u4e4b\u4e00\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4f5c\u4e3adUTP\u7684\u94a0\u76d0\u5f62\u5f0f\uff0c\u8be5\u5316\u5408\u7269\u5728\u7ec6\u80de\u5185\u53c2\u4e0e\u5627\u5576\u4ee3\u8c22\u9014\u5f84\uff0c\u662f\u80f8\u82f7\u9178\u5408\u6210\u9176\uff08TS\uff09\u7684\u5929\u7136\u5e95\u7269\u3002\u901a\u8fc7\u5c3f\u5627\u5576-DNA\u7cd6\u57fa\u5316\u9176\uff08UNG\uff09\u7cfb\u7edf\uff0c\u53ef\u8c03\u63a7DNA\u4e2d\u5c3f\u5627\u5576\u7684\u63ba\u5165\u4e0e\u4fee\u590d\uff0c\u5bf9\u7ef4\u6301\u57fa\u56e0\u7ec4\u7a33\u5b9a\u6027\u81f3\u5173\u91cd\u8981\u3002\u6b64\u5916\uff0c\u5176\u5728PCR\u6269\u589e\u3001DNA\u6807\u8bb0\u7b49\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\u4e2d\u5177\u6709\u4e0d\u53ef\u66ff\u4ee3\u7684\u4f5c\u7528\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u672c\u4ea7\u54c1\u5e7f\u6cdb\u5e94\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u4f5c\u4e3aDNA\u5408\u6210\u539f\u6599\uff1b\u4f53\u5916\u8bca\u65ad\u8bd5\u5242\u5f00\u53d1\u4e2d\u7528\u4e8e\u63a2\u9488\u6807\u8bb0\uff1b\u6297\u75c5\u6bd2\u836f\u7269\u7b5b\u9009\u6a21\u578b\u4e2d\u4f5c\u4e3a\u9176\u53cd\u5e94\u5e95\u7269\uff1b\u8868\u89c2\u9057\u4f20\u5b66\u7814\u7a76\u6d89\u53caDNA\u635f\u4f24\u4e0e\u4fee\u590d\u673a\u5236\u5206\u6790\u3002\u5177\u4f53\u5b9e\u9a8c\u5305\u62ec\uff1aqPCR\/dPCR\u6807\u51c6\u54c1\u5236\u5907\u3001\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08NGS\uff09\u6587\u5e93\u6784\u5efa\u3001DNA\u805a\u5408\u9176\u6d3b\u6027\u6d4b\u5b9a\u7b49\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u63a8\u8350-20\u2103\u5e72\u71e5\u907f\u5149\u4fdd\u5b58\uff0c\u5f00\u5c01\u540e\u9700\u5206\u88c5\u4ee5\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\uff0c\u914d\u5236\u6eb6\u6db2\u5efa\u8bae\u7528\u65e0\u6838\u9178\u9176\u7684\u8d85\u7eaf\u6c34\uff0c\u5de5\u4f5c\u6d53\u5ea6\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\uff08\u5e38\u89c4\u8303\u56f40.1-10 mM\uff09\u3002\u907f\u514d\u4e0e\u5f3a\u6c27\u5316\u5242\u63a5\u89e6\uff0c\u6eb6\u6db2\u73b0\u914d\u73b0\u7528\uff0c\u5269\u4f59\u6db2\u4f53\u9700-80\u2103\u77ed\u671f\u4fdd\u5b58\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6\uff1e96%\uff0c\u5185\u6bd2\u7d20\u68c0\u6d4b\uff1c0.1 EU\/\u03bcg\uff0cDNase\/RNase\u6d3b\u6027\u672a\u68c0\u51fa\u3002\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u88c5\u5907\uff0c\u907f\u514d\u5438\u5165\u6216\u76ae\u80a4\u63a5\u89e6\u3002\u5316\u5b66\u5e9f\u5f03\u7269\u5e94\u6309\u7167\u5371\u9669\u6709\u673a\u7269\u89c4\u8303\u5904\u7f6e\u3002\u5b89\u5168\u6570\u636e\u8868\uff08SDS\uff09\u5df2\u901a\u8fc7ISO 10993-1\u751f\u7269\u76f8\u5bb9\u6027\u8ba4\u8bc1\uff0c\u8fd0\u8f93\u5206\u7c7b\u4e3a\u975e\u9650\u5236\u6027\u5316\u5b66\u54c1\u3002 <\/p>\n
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