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10-\u4e59\u9170\u57fa-3,7-\u4e8c\u7f9f\u57fa\u5429\u55ea\uff08ADHP\uff09\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n

1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n10-\u4e59\u9170\u57fa-3,7-\u4e8c\u7f9f\u57fa\u5429\u55ea\uff08ADHP\uff09\u662f\u4e00\u79cd\u9ad8\u7eaf\u5ea6\u6709\u673a\u5316\u5408\u7269\uff0c\u5316\u5b66\u540d\u79f0\u4e3a10-Acetyl-3,7-dihydroxyphenoxazine\uff0cCAS\u53f7\u4e3a119171-73-2\u3002\u5176\u5206\u5b50\u5f0f\u4e3aC14H11NO4\uff0c\u5206\u5b50\u91cf\u4e3a257.241\uff0c\u7eaf\u5ea6\u6807\u51c6\u5927\u4e8e96%\u3002\u8be5\u5316\u5408\u7269\u4e3a\u6d45\u9ec4\u8272\u81f3\u68d5\u8272\u7ed3\u6676\u7c89\u672b\uff0c\u5177\u6709\u7279\u5b9a\u7684\u8367\u5149\u7279\u6027\uff0c\u6613\u6eb6\u4e8e\u6781\u6027\u6709\u673a\u6eb6\u5242\u5982\u7532\u9187\u3001\u4e59\u9187\u548cDMSO\uff0c\u5fae\u6eb6\u4e8e\u6c34\u3002ADHP\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\u7a33\u5b9a\u6027\u8f83\u9ad8\uff0c\u4f46\u5728\u5f3a\u9178\u6027\u73af\u5883\u4e2d\u53ef\u80fd\u53d1\u751f\u964d\u89e3\u3002 <\/p>\n

2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\nADHP\u662f\u4e00\u79cd\u91cd\u8981\u7684\u8367\u5149\u5e95\u7269\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6c27\u5316\u8fd8\u539f\u53cd\u5e94\u7684\u68c0\u6d4b\u3002\u5176\u6838\u5fc3\u529f\u80fd\u662f\u901a\u8fc7\u8fc7\u6c27\u5316\u7269\u9176\uff08\u5982\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176HRP\uff09\u50ac\u5316\u6c27\u5316\u540e\uff0c\u751f\u6210\u9ad8\u8367\u5149\u4ea7\u7269\u8bd5\u5364\u7075\uff08resorufin\uff09\uff0c\u8367\u5149\u6fc0\u53d1\u6ce2\u957f\u4e3a563 nm\uff0c\u53d1\u5c04\u6ce2\u957f\u4e3a587 nm\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u68c0\u6d4b\u8fc7\u6c27\u5316\u6c22\uff08H2O2\uff09\u548c\u8fc7\u6c27\u5316\u7269\u9176\u6d3b\u6027\u7684\u9ad8\u7075\u654f\u5ea6\u5de5\u5177\u3002ADHP\u7684\u68c0\u6d4b\u9650\u4f4e\u81f3\u7eb3\u6469\u5c14\u7ea7\u522b\uff0c\u5728\u751f\u7269\u533b\u5b66\u7814\u7a76\u548c\u4e34\u5e8a\u8bca\u65ad\u4e2d\u5177\u6709\u4e0d\u53ef\u66ff\u4ee3\u7684\u4f5c\u7528\u3002 <\/p>\n

3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\nADHP\u4e3b\u8981\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a
\n– \u751f\u7269\u4f20\u611f\u5668\u5f00\u53d1\uff1a\u4f5c\u4e3aH2O2\u68c0\u6d4b\u7684\u6838\u5fc3\u8bd5\u5242\uff0c\u7528\u4e8e\u8461\u8404\u7cd6\u3001\u80c6\u56fa\u9187\u7b49\u4ee3\u8c22\u7269\u7684\u9176\u6cd5\u5206\u6790\u3002
\n– \u7ec6\u80de\u6d3b\u6027\u68c0\u6d4b\uff1a\u5728\u7ec6\u80de\u51cb\u4ea1\u3001\u6c27\u5316\u5e94\u6fc0\u7814\u7a76\u4e2d\u7528\u4e8e\u91cf\u5316\u6d3b\u6027\u6c27\uff08ROS\uff09\u6c34\u5e73\u3002
\n– \u836f\u7269\u7b5b\u9009\uff1a\u8bc4\u4f30\u6297\u6c27\u5316\u836f\u7269\u6216\u5316\u5408\u7269\u7684\u6d3b\u6027\u3002
\n– \u73af\u5883\u76d1\u6d4b\uff1a\u68c0\u6d4b\u6c34\u4f53\u6216\u7a7a\u6c14\u4e2d\u7684\u8fc7\u6c27\u5316\u7269\u6c61\u67d3\u3002 <\/p>\n

4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\nADHP\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u957f\u671f\u50a8\u5b58\u5efa\u8bae\u5145\u6c2e\u4fdd\u62a4\u3002\u5f00\u5c01\u540e\u9700\u5206\u88c5\u4f7f\u7528\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5de5\u4f5c\u6db2\u5efa\u8bae\u73b0\u914d\u73b0\u7528\uff0c\u6eb6\u5242\u63a8\u8350\u4f7f\u7528\u65e0\u6c34DMSO\u6216\u4e59\u9187\uff0c\u914d\u5236\u540e4\u00b0C\u4fdd\u5b58\u4e0d\u8d85\u8fc724\u5c0f\u65f6\u3002\u5b9e\u9a8c\u64cd\u4f5c\u9700\u5728\u5f31\u78b1\u6027\uff08pH 7-8\uff09\u7f13\u51b2\u4f53\u7cfb\u4e2d\u8fdb\u884c\u4ee5\u4f18\u5316\u53cd\u5e94\u6548\u7387\u3002 <\/p>\n

5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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