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\u4e09\u78f7\u9178\u5421\u5576\u6838\u82f7\u9178\u94a0\u76d0\u6c34\u5408\u7269\u4ea7\u54c1\u8bf4\u660e <\/p>\n

1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u4e09\u78f7\u9178\u5421\u5576\u6838\u82f7\u9178\u94a0\u76d0\u6c34\u5408\u7269\uff08\u5316\u5b66\u540d\u79f0\uff1asodium,[[5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-oxidophosphoryl] [5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate\uff09\u662f\u4e00\u79cd\u91cd\u8981\u7684\u8f85\u9176\u7c7b\u5316\u5408\u7269\uff0cCAS\u53f7\u4e3a698999-85-8\uff0c\u5206\u5b50\u5f0f\u4e3aC21H27N7NaO17P3\uff0c\u5206\u5b50\u91cf\u4e3a765.387\u3002\u672c\u54c1\u4ee5\u94a0\u76d0\u5f62\u5f0f\u5b58\u5728\uff0c\u7eaf\u5ea6>96%\uff0c\u5916\u89c2\u901a\u5e38\u4e3a\u767d\u8272\u6216\u7c7b\u767d\u8272\u7c89\u672b\uff0c\u6613\u6eb6\u4e8e\u6c34\uff0c\u5728\u751f\u7269\u5316\u5b66\u7814\u7a76\u4e2d\u5177\u6709\u5e7f\u6cdb\u7528\u9014\u3002 <\/p>\n

2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4e09\u78f7\u9178\u5421\u5576\u6838\u82f7\u9178\uff08NADP+\uff09\u662f\u70df\u9170\u80fa\u817a\u560c\u5464\u4e8c\u6838\u82f7\u9178\u78f7\u9178\u7684\u6c27\u5316\u5f62\u5f0f\uff0c\u4f5c\u4e3a\u5173\u952e\u8f85\u9176\u53c2\u4e0e\u591a\u79cd\u6c27\u5316\u8fd8\u539f\u53cd\u5e94\u3002\u5176\u5728\u751f\u7269\u4f53\u5185\u7684\u8fd8\u539f\u5f62\u5f0fNADPH\u662f\u91cd\u8981\u7684\u8fd8\u539f\u529b\u4f9b\u4f53\uff0c\u4e3a\u8102\u80aa\u9178\u5408\u6210\u3001\u80c6\u56fa\u9187\u4ee3\u8c22\u548c\u6297\u6c27\u5316\u9632\u5fa1\u7cfb\u7edf\u63d0\u4f9b\u5fc5\u9700\u7684\u7535\u5b50\u3002\u6b64\u5916\uff0cNADP+\u5728\u5149\u5408\u4f5c\u7528\u3001\u7ec6\u80de\u4fe1\u53f7\u4f20\u5bfc\u548cDNA\u4fee\u590d\u7b49\u8fc7\u7a0b\u4e2d\u4e5f\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002 <\/p>\n

3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u672c\u54c1\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5316\u5b66\u548c\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u9886\u57df\uff0c\u5177\u4f53\u7528\u9014\u5305\u62ec\uff1a
\n– \u4f5c\u4e3a\u9176\u5b66\u7814\u7a76\u7684\u5e95\u7269\u6216\u8f85\u56e0\u5b50\uff0c\u7528\u4e8e\u6d4b\u5b9a\u8131\u6c22\u9176\u6d3b\u6027\uff1b
\n– \u7528\u4e8e\u7ec6\u80de\u4ee3\u8c22\u7814\u7a76\uff0c\u7279\u522b\u662f\u80fd\u91cf\u4ee3\u8c22\u548c\u6c27\u5316\u8fd8\u539f\u5e73\u8861\u5206\u6790\uff1b
\n– \u5728\u836f\u7269\u7814\u53d1\u4e2d\u7528\u4e8e\u7b5b\u9009\u5f71\u54cdNADPH\u4f9d\u8d56\u6027\u901a\u8def\u7684\u5316\u5408\u7269\uff1b
\n– \u4f5c\u4e3a\u8bca\u65ad\u8bd5\u5242\u7ec4\u5206\uff0c\u7528\u4e8e\u4e34\u5e8a\u751f\u5316\u68c0\u6d4b\u3002 <\/p>\n

4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u9700\u4e25\u683c\u5728-20\u00b0C\u4e0b\u907f\u5149\u5e72\u71e5\u4fdd\u5b58\uff0c\u5f00\u5c01\u540e\u5efa\u8bae\u5206\u88c5\u4ee5\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\uff0c\u6eb6\u89e3\u65f6\u5efa\u8bae\u4f7f\u7528\u65e0\u83cc\u7f13\u51b2\u6db2\uff08\u5982Tris-HCl\u6216PBS\uff09\u3002\u6eb6\u6db2\u72b6\u6001\u4e0d\u7a33\u5b9a\uff0c\u5efa\u8bae\u73b0\u914d\u73b0\u7528\u3002\u907f\u514d\u4e0e\u5f3a\u9178\u3001\u5f3a\u78b1\u6216\u6c27\u5316\u5242\u63a5\u89e6\u3002 <\/p>\n

5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecfHPLC\u68c0\u6d4b\u7eaf\u5ea6>96%\uff0c\u5e76\u7b26\u5408\u751f\u7269\u5b66\u7814\u7a76\u7ea7\u6807\u51c6\u3002\u5b89\u5168\u4fe1\u606f\uff1a
\n– \u672c\u54c1\u5bf9\u773c\u775b\u548c\u76ae\u80a4\u6709\u8f7b\u5fae\u523a\u6fc0\u6027\uff0c\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u88c5\u5907\uff1b
\n– \u82e5\u8bef\u63a5\u89e6\uff0c\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u5e76\u5c31\u533b\uff1b
\n– \u5e9f\u5f03\u7269\u9700\u6309\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\uff1b
\n– \u50a8\u5b58\u548c\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u9700\u9075\u5b88\u5b9e\u9a8c\u5ba4\u5b89\u5168\u89c4\u7a0b\u3002 <\/p>\n

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