11-\u53e0\u6c2e\u57fa-3,6,9-\u4e09\u6c27\u4ee3\u5341\u4e00\u9178\uff0811-Azido-3,6,9-trioxaundecanoic Acid\uff09\u662f\u4e00\u79cd\u542b\u53e0\u6c2e\u57fa\u56e2\u7684\u529f\u80fd\u5316\u805a\u4e59\u4e8c\u9187\uff08PEG\uff09\u884d\u751f\u7269\uff0cCAS\u53f7\u4e3a172531-37-2\uff0c\u5206\u5b50\u5f0f\u4e3aC8H15N3O5\uff0c\u5206\u5b50\u91cf233.222\u3002\u8be5\u5316\u5408\u7269\u7531\u4eb2\u6c34\u6027PEG\u94fe\u3001\u672b\u7aef\u7fa7\u9178\u57fa\u56e2\u53ca\u53e0\u6c2e\u57fa\u56e2\u6784\u6210\uff0c\u517c\u5177\u6c34\u6eb6\u6027\u4e0e\u53cd\u5e94\u6d3b\u6027\uff0c\u7eaf\u5ea6>96%\uff0c\u5e38\u6e29\u4e0b\u4e3a\u65e0\u8272\u81f3\u6de1\u9ec4\u8272\u6db2\u4f53\u6216\u4f4e\u7194\u70b9\u56fa\u4f53\u3002<\/p>\n
1. \u5316\u5b66\u7279\u6027\u4e0e\u529f\u80fd
\n\u8be5\u5206\u5b50\u6838\u5fc3\u7ed3\u6784\u4e3a\u4e09\u4e59\u4e8c\u9187\u5355\u5143\uff08-3,6,9-trioxa-\uff09\uff0c\u8d4b\u4e88\u5176\u4f18\u5f02\u7684\u6c34\u6eb6\u6027\u548c\u751f\u7269\u76f8\u5bb9\u6027\u3002\u672b\u7aef\u7684\u53e0\u6c2e\u57fa\uff08-N3\uff09\u53ef\u901a\u8fc7\u70b9\u51fb\u5316\u5b66\uff08\u5982CuAAC\u53cd\u5e94\uff09\u4e0e\u7094\u70c3\u9ad8\u6548\u5076\u8054\uff0c\u7fa7\u9178\u57fa\u56e2\u5219\u4fbf\u4e8e\u4e0e\u6c28\u57fa\u5f62\u6210\u9170\u80fa\u952e\u3002\u8fd9\u79cd\u53cc\u529f\u80fd\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u751f\u7269\u5171\u4ef7\u8fde\u63a5\u7684\u7406\u60f3 linker\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u86cb\u767d\u8d28\u4fee\u9970\u3001\u836f\u7269\u9012\u9001\u7cfb\u7edf\u6784\u5efa\u53ca\u6750\u6599\u8868\u9762\u529f\u80fd\u5316\u3002<\/p>\n
2. \u751f\u7269\u5316\u5b66\u5e94\u7528
\n\u5728\u751f\u7269\u5076\u8054\u9886\u57df\uff0c\u672c\u54c1\u5e38\u7528\u4e8e\u6297\u4f53-\u836f\u7269\u5076\u8054\u7269\uff08ADCs\uff09\u3001\u8367\u5149\u6807\u8bb0\u63a2\u9488\u548c\u7eb3\u7c73\u8f7d\u4f53\u5f00\u53d1\u3002\u53e0\u6c2e\u57fa\u56e2\u7684\u9ad8\u9009\u62e9\u6027\u53cd\u5e94\u53ef\u907f\u514d\u751f\u7269\u5206\u5b50\u4ea4\u8054\u526f\u53cd\u5e94\uff0cPEG\u94fe\u80fd\u51cf\u5c11\u514d\u75ab\u539f\u6027\u5e76\u5ef6\u957f\u5faa\u73af\u534a\u8870\u671f\u3002\u4f8b\u5982\uff0c\u901a\u8fc7\u7fa7\u57fa\u6d3b\u5316\u5c06\u836f\u7269\u5206\u5b50\u5171\u4ef7\u8fde\u63a5\u540e\uff0c\u53e0\u6c2e\u7aef\u53ef\u4e0e\u9776\u5411\u5206\u5b50\u7684\u7094\u57fa\u884d\u751f\u7269\u70b9\u51fb\u53cd\u5e94\uff0c\u5b9e\u73b0\u7cbe\u51c6\u5076\u8054\u3002<\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u573a\u666f
\n3.1 \u751f\u7269\u6807\u8bb0\uff1a\u7528\u4e8e\u86cb\u767d\u8d28\u3001\u6838\u9178\u7684\u8367\u5149\u6807\u8bb0\u6216\u751f\u7269\u7d20\u5316
\n3.2 \u836f\u7269\u5f00\u53d1\uff1a\u6784\u5efaADC\u836f\u7269\u4e2d\u7684PEG\u5316\u8fde\u63a5\u81c2
\n3.3 \u6750\u6599\u79d1\u5b66\uff1a\u4fee\u9970\u805a\u5408\u7269\u6216\u7eb3\u7c73\u9897\u7c92\u8868\u9762\u4ee5\u5f15\u5165\u751f\u7269\u6d3b\u6027\u57fa\u56e2
\n3.4 \u8bca\u65ad\u8bd5\u5242\uff1a\u5f00\u53d1\u514d\u75ab\u68c0\u6d4b\u4e2d\u7684\u4fe1\u53f7\u653e\u5927\u7cfb\u7edf <\/p>\n
4. \u50a8\u5b58\u4e0e\u64cd\u4f5c
\n\u5efa\u8bae\u907f\u5149\u4fdd\u5b58\u4e8e-20\u2103\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u5f00\u5c01\u540e\u9700\u5145\u60f0\u6027\u6c14\u4f53\u4fdd\u62a4\u3002\u6eb6\u89e3\u65f6\u4f18\u5148\u9009\u7528DMF\u3001DMSO\u6216PBS\u7f13\u51b2\u6db2\uff08pH 7-8\uff09\u3002\u53e0\u6c2e\u57fa\u56e2\u53d7\u70ed\u6216\u9707\u52a8\u53ef\u80fd\u5206\u89e3\uff0c\u64cd\u4f5c\u65f6\u5e94\u4f69\u6234\u9632\u62a4\u88c5\u5907\u5e76\u5728\u901a\u98ce\u6a71\u4e2d\u8fdb\u884c\u3002<\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168
\n\u672c\u4ea7\u54c1\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6>96%\uff0cMS\u548cNMR\u786e\u8ba4\u7ed3\u6784\u3002\u9700\u6ce8\u610f\u53e0\u6c2e\u5316\u5408\u7269\u53ef\u80fd\u5177\u7206\u70b8\u6027\uff0c\u907f\u514d\u4e0e\u8fd8\u539f\u6027\u7269\u8d28\u3001\u91cd\u91d1\u5c5e\u6216\u5f3a\u9178\u63a5\u89e6\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u5371\u9669\u5316\u5b66\u54c1\u5904\u7406\uff0c\u76ae\u80a4\u63a5\u89e6\u540e\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u3002\u5efa\u8bae\u5b9e\u9a8c\u524d\u67e5\u9605MSDS\u83b7\u53d6\u8be6\u7ec6\u5b89\u5168\u6570\u636e\u3002 <\/p>\n
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