N-Biotinylaminoethyl Methanethiosulfonate \u4ea7\u54c1\u8bf4\u660e <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\nN-Biotinylaminoethyl Methanethiosulfonate\uff08CAS\u53f7\uff1a162758-04-5\uff09\u662f\u4e00\u79cd\u751f\u7269\u7d20\u5316\u7684\u786b\u9187\u53cd\u5e94\u6027\u8bd5\u5242\uff0c\u5206\u5b50\u5f0f\u4e3aC13H23N3O4S3\uff0c\u5206\u5b50\u91cf\u4e3a381.534\u3002\u8be5\u5316\u5408\u7269\u7531\u751f\u7269\u7d20\u57fa\u56e2\u901a\u8fc7\u6c28\u57fa\u4e59\u57fa\u8fde\u63a5\u81c2\u4e0e\u7532\u786b\u4ee3\u78fa\u9178\u916f\u57fa\u56e2\u6784\u6210\uff0c\u5177\u6709\u9ad8\u53cd\u5e94\u6d3b\u6027\u548c\u7279\u5f02\u6027\u3002\u5176\u7eaf\u5ea6\u7ecf\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\u9a8c\u8bc1\u5927\u4e8e96%\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u53ef\u9760\u6027\u548c\u91cd\u590d\u6027\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u8be5\u8bd5\u5242\u7684\u6838\u5fc3\u529f\u80fd\u662f\u901a\u8fc7\u7532\u786b\u4ee3\u78fa\u9178\u916f\u57fa\u56e2\u4e0e\u86cb\u767d\u8d28\u6216\u5176\u4ed6\u751f\u7269\u5206\u5b50\u4e2d\u7684\u6e38\u79bb\u5def\u57fa\uff08-SH\uff09\u53d1\u751f\u7279\u5f02\u6027\u53cd\u5e94\uff0c\u5f62\u6210\u7a33\u5b9a\u7684\u4e8c\u786b\u952e\u8fde\u63a5\u3002\u540c\u65f6\uff0c\u5176\u751f\u7269\u7d20\u6807\u7b7e\u53ef\u901a\u8fc7\u94fe\u9709\u4eb2\u548c\u7d20\u6216\u4eb2\u548c\u7d20\u7cfb\u7edf\u5b9e\u73b0\u9ad8\u6548\u68c0\u6d4b\u6216\u5206\u79bb\u3002\u8fd9\u79cd\u53cc\u91cd\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u86cb\u767d\u8d28\u6807\u8bb0\u3001\u7eaf\u5316\u548c\u68c0\u6d4b\u7684\u91cd\u8981\u5de5\u5177\uff0c\u5c24\u5176\u5728\u7814\u7a76\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u3001\u7ec6\u80de\u8868\u9762\u6807\u8bb0\u548c\u4fe1\u53f7\u8f6c\u5bfc\u4e2d\u5177\u6709\u4e0d\u53ef\u66ff\u4ee3\u7684\u4f5c\u7528\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\nN-Biotinylaminoethyl Methanethiosulfonate\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5316\u5b66\u548c\u5206\u5b50\u751f\u7269\u5b66\u9886\u57df\u3002\u5177\u4f53\u7528\u9014\u5305\u62ec\uff1a
\n– \u86cb\u767d\u8d28\u751f\u7269\u7d20\u5316\u6807\u8bb0\uff1a\u7528\u4e8eWestern blot\u3001ELISA\u6216\u8d28\u8c31\u5206\u6790\u4e2d\u7684\u76ee\u6807\u86cb\u767d\u68c0\u6d4b\u3002
\n– \u7ec6\u80de\u819c\u86cb\u767d\u7814\u7a76\uff1a\u901a\u8fc7\u6807\u8bb0\u7ec6\u80de\u8868\u9762\u5def\u57fa\uff0c\u7814\u7a76\u53d7\u4f53\u5206\u5e03\u548c\u52a8\u6001\u53d8\u5316\u3002
\n– \u4eb2\u548c\u7eaf\u5316\uff1a\u7ed3\u5408\u94fe\u9709\u4eb2\u548c\u7d20\u78c1\u73e0\u6216\u743c\u8102\u7cd6\u73e0\uff0c\u5b9e\u73b0\u76ee\u6807\u5206\u5b50\u7684\u5feb\u901f\u5206\u79bb\u3002
\n– \u7ed3\u6784\u751f\u7269\u5b66\uff1a\u8f85\u52a9\u86cb\u767d\u8d28\u4ea4\u8054\u5b9e\u9a8c\uff0c\u89e3\u6790\u86cb\u767d\u8d28\u590d\u5408\u7269\u6784\u8c61\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u5f00\u5c01\u540e\u5efa\u8bae\u5206\u88c5\u4ee5\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u65f6\u9700\u6eb6\u89e3\u4e8e\u65e0\u6c34DMSO\u6216\u7f13\u51b2\u6db2\uff08\u5982PBS\uff0cpH 7.4\uff09\uff0c\u5e76\u907f\u514d\u63a5\u89e6\u8fd8\u539f\u5242\uff08\u5982DTT\u6216\u03b2-\u5def\u57fa\u4e59\u9187\uff09\u3002\u63a8\u8350\u5de5\u4f5c\u6d53\u5ea6\u4e3a0.1-1 mM\uff0c\u5177\u4f53\u9700\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u6bcf\u6279\u6b21\u4ea7\u54c1\u5747\u901a\u8fc7HPLC\u548c\u8d28\u8c31\u5206\u6790\u9a8c\u8bc1\u7eaf\u5ea6\u548c\u7ed3\u6784\u3002\u64cd\u4f5c\u65f6\u9700\u7a7f\u6234\u9632\u62a4\u88c5\u5907\uff08\u624b\u5957\u3001\u62a4\u76ee\u955c\uff09\uff0c\u907f\u514d\u5438\u5165\u6216\u76ae\u80a4\u63a5\u89e6\u3002\u672c\u54c1\u5bf9\u6c34\u5206\u654f\u611f\uff0c\u9700\u5728\u60f0\u6027\u6c14\u4f53\u4fdd\u62a4\u4e0b\u50a8\u5b58\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u7167\u6709\u673a\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002 <\/p>\n
\u672c\u4ea7\u54c1\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\uff0c\u4e0d\u9002\u7528\u4e8e\u4e34\u5e8a\u6216\u8bca\u65ad\u7528\u9014\u3002<\/p>\n","protected":false},"featured_media":27474,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[25],"class_list":["post-48132","chemical-product","type-chemical-product","status-publish","has-post-thumbnail","hentry","product_category-25"],"acf":[],"taxonomy_info":{"product_category":[{"value":25,"label":"\u5b9e\u9a8c\u836f\u54c1"}]},"featured_image_src_large":["https:\/\/bio-get.com\/wp-content\/uploads\/2026\/04\/162758-04-5.jpg",464,464,false],"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/48132","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/media\/27474"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=48132"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=48132"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}