\u4ee5\u4e0b\u662f\u6839\u636e\u60a8\u7684\u8981\u6c42\u64b0\u5199\u7684\u4e13\u4e1a\u4ea7\u54c1\u8bf4\u660e\uff1a<\/p>\n
\u4ea7\u54c1\u540d\u79f0\uff1a(2R)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-3-(4-nitrophenyl)propanoic acid\uff08Fmoc-D-\u5bf9\u785d\u57fa\u82ef\u4e19\u6c28\u9178\uff09<\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u672c\u4ea7\u54c1\u4e3aFmoc\u4fdd\u62a4\u7684D\u578b\u5bf9\u785d\u57fa\u82ef\u4e19\u6c28\u9178\u884d\u751f\u7269\uff0cCAS\u53f7177966-63-1\uff0c\u5206\u5b50\u5f0fC24H20N2O6\uff0c\u5206\u5b50\u91cf432.425\u3002\u8be5\u5316\u5408\u7269\u662f\u767d\u8272\u81f3\u6de1\u9ec4\u8272\u7ed3\u6676\u6027\u7c89\u672b\uff0c\u7eaf\u5ea6>96%\uff0c\u5177\u6709\u5178\u578b\u7684\u624b\u6027\u4e2d\u5fc3\uff08R\u6784\u578b\uff09\u548c\u5149\u654f\u6027\u785d\u57fa\u82ef\u57fa\u56e2\u3002\u5176\u7ed3\u6784\u4e2d\u7684Fmoc\u4fdd\u62a4\u57fa\uff089-\u82b4\u7532\u6c27\u7fb0\u57fa\uff09\u5728\u5f31\u78b1\u6027\u6761\u4ef6\u4e0b\u53ef\u88ab\u8131\u9664\uff0c\u800c\u7fa7\u9178\u57fa\u56e2\u53ef\u53c2\u4e0e\u7f29\u5408\u53cd\u5e94\u3002<\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4f5c\u4e3a\u975e\u5929\u7136\u6c28\u57fa\u9178\u884d\u751f\u7269\uff0c\u8be5\u4ea7\u54c1\u5728\u80bd\u94fe\u5408\u6210\u4e2d\u5177\u6709\u5173\u952e\u4f5c\u7528\u3002D\u578b\u6784\u578b\u8d4b\u4e88\u5176\u6297\u86cb\u767d\u9176\u964d\u89e3\u7279\u6027\uff0c\u800c\u5bf9\u785d\u57fa\u82ef\u57fa\u589e\u5f3a\u4e86\u7d2b\u5916\u68c0\u6d4b\u7075\u654f\u5ea6\u3002Fmoc\u4fdd\u62a4\u57fa\u56e2\u4f7f\u5176\u6210\u4e3a\u56fa\u76f8\u80bd\u5408\u6210\uff08SPPS\uff09\u7684\u7406\u60f3\u6784\u5efa\u5355\u5143\uff0c\u7279\u522b\u9002\u7528\u4e8e\u9700\u8981\u5f15\u5165\u7279\u6b8a\u5b98\u80fd\u56e2\u7684\u80bd\u6bb5\u8bbe\u8ba1\u3002<\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u4e3b\u8981\u5e94\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a1\uff09\u591a\u80bd\u836f\u7269\u7814\u53d1\u4e2d\u4f5c\u4e3a\u7ed3\u6784\u4fee\u9970\u5355\u5143\uff1b2\uff09\u751f\u7269\u6807\u8bb0\u7269\u5408\u6210\u4e2d\u7684\u8367\u5149\u63a2\u9488\u524d\u4f53\uff1b3\uff09\u9176\u6291\u5236\u5242\u8bbe\u8ba1\u7684\u6838\u5fc3\u9aa8\u67b6\uff1b4\uff09\u4e0d\u5bf9\u79f0\u50ac\u5316\u7814\u7a76\u7684\u624b\u6027\u6a21\u677f\u3002\u5177\u4f53\u7528\u4e8e\u6784\u5efa\u542b\u6709\u785d\u57fa\u82ef\u57fa\u7684\u7279\u5f02\u6027\u80bd\u5e8f\u5217\uff0c\u6216\u4f5c\u4e3a\u86cb\u767d\u8d28-\u5c0f\u5206\u5b50\u76f8\u4e92\u4f5c\u7528\u7814\u7a76\u7684\u5206\u5b50\u5de5\u5177\u3002<\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u5efa\u8bae\u50a8\u5b58\u6761\u4ef6\uff1a-20\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u5e72\u71e5\u60f0\u6027\u6c14\u4f53\u73af\u5883\u4e0b\u5bc6\u5c01\u3002\u4f7f\u7528\u524d\u9700\u6062\u590d\u81f3\u5ba4\u6e29\u5e76\u4fdd\u6301\u5e72\u71e5\uff0c\u907f\u514d\u4e0e\u5f3a\u6c27\u5316\u5242\u63a5\u89e6\u3002\u6eb6\u89e3\u63a8\u8350\u4f7f\u7528DMF\u6216DMSO\u7b49\u6781\u6027\u975e\u8d28\u5b50\u6eb6\u5242\uff0c\u5de5\u4f5c\u6d53\u5ea6\u6839\u636e\u5b9e\u9a8c\u9700\u6c42\u914d\u7f6e\uff08\u901a\u5e381-10mM\uff09\u3002\u8131\u4fdd\u62a4\u5efa\u8bae\u4f7f\u752820%\u54cc\u5576\/DMF\u6eb6\u6db2\u3002<\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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