4-(\u7f9f\u57fa\u6c28\u57fa)\u55b9\u5549 N-\u6c27\u5316\u7269\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n4-(\u7f9f\u57fa\u6c28\u57fa)\u55b9\u5549 N-\u6c27\u5316\u7269\uff084-(hydroxyamino)quinoline N-oxide\uff09\u662f\u4e00\u79cd\u55b9\u5549\u7c7b\u884d\u751f\u7269\uff0c\u5316\u5b66\u5f0f\u4e3aC9H8N2O2\uff0c\u5206\u5b50\u91cf176.172\uff0cCAS\u53f7\u4e3a4637-56-3\u3002\u672c\u54c1\u4e3a\u9ec4\u8272\u81f3\u68d5\u8272\u7ed3\u6676\u6027\u7c89\u672b\uff0c\u7eaf\u5ea6\u9ad8\u4e8e96%\uff0c\u53ef\u6eb6\u4e8e\u6709\u673a\u6eb6\u5242\u5982DMSO\u548c\u7532\u9187\uff0c\u5fae\u6eb6\u4e8e\u6c34\u3002\u5176\u7ed3\u6784\u4e2d\u7684\u7f9f\u57fa\u6c28\u57fa\u548cN-\u6c27\u5316\u7269\u57fa\u56e2\u8d4b\u4e88\u5176\u72ec\u7279\u7684\u5316\u5b66\u6027\u8d28\uff0c\u4f7f\u5176\u5728\u751f\u7269\u5316\u5b66\u7814\u7a76\u4e2d\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u8be5\u5316\u5408\u7269\u5728\u751f\u7269\u5316\u5b66\u7814\u7a76\u4e2d\u4e3b\u8981\u4f5c\u4e3a\u6d3b\u6027\u6c27\u81ea\u7531\u57fa\uff08ROS\uff09\u7684\u751f\u6210\u5242\u548cDNA\u635f\u4f24\u8bf1\u5bfc\u5242\u3002\u5176N-\u6c27\u5316\u7269\u7ed3\u6784\u5728\u8fd8\u539f\u6761\u4ef6\u4e0b\u53ef\u4ea7\u751f\u6d3b\u6027\u4e2d\u95f4\u4f53\uff0c\u8fdb\u800c\u4e0e\u751f\u7269\u5927\u5206\u5b50\u5982DNA\u3001\u86cb\u767d\u8d28\u53d1\u751f\u76f8\u4e92\u4f5c\u7528\uff0c\u6a21\u62df\u6c27\u5316\u5e94\u6fc0\u73af\u5883\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u7814\u7a76\u6c27\u5316\u635f\u4f24\u673a\u5236\u3001\u7ec6\u80de\u51cb\u4ea1\u548c\u81f4\u764c\u8fc7\u7a0b\u7684\u91cd\u8981\u5de5\u5177\u5206\u5b50\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n4-(\u7f9f\u57fa\u6c28\u57fa)\u55b9\u5549 N-\u6c27\u5316\u7269\u5e7f\u6cdb\u5e94\u7528\u4e8e\u80bf\u7624\u5b66\u7814\u7a76\u3001\u6bd2\u7406\u5b66\u8bc4\u4f30\u548c\u81ea\u7531\u57fa\u751f\u7269\u5b66\u9886\u57df\u3002\u5177\u4f53\u7528\u9014\u5305\u62ec\uff1a\u4f5c\u4e3a\u4f53\u5916\u5b9e\u9a8c\u4e2d\u7684DNA\u635f\u4f24\u5242\uff0c\u7528\u4e8e\u7814\u7a76\u4fee\u590d\u673a\u5236\uff1b\u5728\u7ec6\u80de\u6a21\u578b\u4e2d\u8bf1\u5bfc\u6c27\u5316\u5e94\u6fc0\uff0c\u8bc4\u4f30\u6297\u6c27\u5316\u5242\u7684\u4fdd\u62a4\u4f5c\u7528\uff1b\u4f5c\u4e3a\u6807\u51c6\u54c1\u7528\u4e8e\u5206\u6790\u68c0\u6d4b\u65b9\u6cd5\u7684\u5f00\u53d1\u4e0e\u9a8c\u8bc1\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u957f\u671f\u50a8\u5b58\u5efa\u8bae\u5145\u6c2e\u4fdd\u62a4\u3002\u5f00\u5c01\u540e\u9700\u5bc6\u5c01\u9632\u6f6e\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u65f6\u9700\u5728\u901a\u98ce\u6a71\u4e2d\u64cd\u4f5c\uff0c\u4f69\u6234\u9632\u62a4\u624b\u5957\u548c\u62a4\u76ee\u955c\u3002\u5efa\u8bae\u7528DMSO\u914d\u5236\u6bcd\u6db2\uff0c\u5de5\u4f5c\u6d53\u5ea6\u9700\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\uff0c\u907f\u514d\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u6216\u5438\u5165\u7c89\u5c18\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecfHPLC\u68c0\u6d4b\u7eaf\u5ea6\u226596%\uff0c\u6279\u6b21\u95f4\u7a33\u5b9a\u6027\u4e25\u683c\u63a7\u5236\u3002\u5b89\u5168\u6570\u636e\u8868\u660e\uff0c\u8be5\u5316\u5408\u7269\u5177\u6709\u523a\u6fc0\u6027\uff0c\u53ef\u80fd\u5f15\u8d77\u76ae\u80a4\u3001\u773c\u775b\u548c\u547c\u5438\u9053\u4e0d\u9002\u3002\u64cd\u4f5c\u65f6\u5e94\u9075\u5b88\u5b9e\u9a8c\u5ba4\u5b89\u5168\u89c4\u8303\uff0c\u5982\u610f\u5916\u63a5\u89e6\u9700\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u5e76\u5c31\u533b\u3002\u5e9f\u5f03\u7269\u9700\u6309\u5371\u9669\u5316\u5b66\u54c1\u5904\u7406\uff0c\u7981\u6b62\u76f4\u63a5\u6392\u653e\u81f3\u73af\u5883\u4e2d\u3002 <\/p>\n
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