RG 108\uff08N-\u915e\u9170-L-\u8272\u6c28\u9178\uff09\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\nRG 108\u662f\u4e00\u79cd\u5c0f\u5206\u5b50\u5316\u5408\u7269\uff0c\u5316\u5b66\u540d\u79f0\u4e3aN-\u915e\u9170-L-\u8272\u6c28\u9178\uff0cCAS\u53f7\u4e3a48208-26-0\u3002\u5176\u5206\u5b50\u5f0f\u4e3aC19H14N2O4\uff0c\u5206\u5b50\u91cf\u4e3a334.326\uff0c\u7eaf\u5ea6\u9ad8\u4e8e96%\u3002\u8be5\u5316\u5408\u7269\u4e3a\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7c89\u672b\uff0c\u53ef\u6eb6\u4e8e\u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u548c\u7532\u9187\uff0c\u4f46\u5728\u6c34\u4e2d\u7684\u6eb6\u89e3\u5ea6\u8f83\u4f4e\u3002\u5176\u7ed3\u6784\u5305\u542b\u915e\u9170\u57fa\u548c\u8272\u6c28\u9178\u6b8b\u57fa\uff0c\u5177\u6709\u7279\u5b9a\u7684\u7acb\u4f53\u6784\u578b\u548c\u751f\u7269\u6d3b\u6027\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\nRG 108\u662f\u4e00\u79cdDNA\u7532\u57fa\u8f6c\u79fb\u9176\uff08DNMT\uff09\u6291\u5236\u5242\uff0c\u901a\u8fc7\u9009\u62e9\u6027\u6291\u5236DNMT1\u548cDNMT3a\u7684\u6d3b\u6027\uff0c\u964d\u4f4e\u57fa\u56e0\u7ec4DNA\u7684\u7532\u57fa\u5316\u6c34\u5e73\u3002\u8fd9\u4e00\u673a\u5236\u4f7f\u5176\u6210\u4e3a\u8868\u89c2\u9057\u4f20\u5b66\u7814\u7a76\u4e2d\u7684\u91cd\u8981\u5de5\u5177\u5206\u5b50\uff0c\u5c24\u5176\u5728DNA\u53bb\u7532\u57fa\u5316\u8c03\u63a7\u3001\u57fa\u56e0\u6c89\u9ed8\u9006\u8f6c\u548c\u5e72\u7ec6\u80de\u91cd\u7f16\u7a0b\u7b49\u9886\u57df\u5177\u6709\u5173\u952e\u4f5c\u7528\u3002\u5176\u9ad8\u6548\u6027\u548c\u76f8\u5bf9\u4f4e\u6bd2\u6027\u4f7f\u5176\u4f18\u4e8e\u4f20\u7edf\u53bb\u7532\u57fa\u5316\u8bd5\u5242\u59825-\u6c2e\u6742\u80de\u82f7\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\nRG 108\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u7840\u7814\u7a76\u548c\u836f\u7269\u5f00\u53d1\u9886\u57df\u3002\u5728\u764c\u75c7\u7814\u7a76\u4e2d\uff0c\u5b83\u7528\u4e8e\u63a2\u7a76DNA\u7532\u57fa\u5316\u4e0e\u80bf\u7624\u53d1\u751f\u7684\u5173\u7cfb\uff1b\u5728\u518d\u751f\u533b\u5b66\u4e2d\uff0c\u5b83\u53ef\u4fc3\u8fdb\u4f53\u7ec6\u80de\u91cd\u7f16\u7a0b\u4e3a\u8bf1\u5bfc\u591a\u80fd\u5e72\u7ec6\u80de\uff08iPSCs\uff09\uff1b\u6b64\u5916\uff0c\u5b83\u8fd8\u7528\u4e8e\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u6a21\u578b\u6784\u5efa\u548c\u8868\u89c2\u9057\u4f20\u836f\u7269\u7b5b\u9009\u3002\u5178\u578b\u5b9e\u9a8c\u6d53\u5ea6\u8303\u56f4\u4e3a5-20 \u03bcM\uff0c\u5177\u4f53\u9700\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u548c\u5b9e\u9a8c\u8bbe\u8ba1\u4f18\u5316\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u957f\u671f\u50a8\u5b58\u5efa\u8bae\u5145\u5165\u60f0\u6027\u6c14\u4f53\u3002\u4f7f\u7528\u524d\u9700\u5ba4\u6e29\u5e73\u8861\u4ee5\u907f\u514d\u5438\u6e7f\uff0c\u6eb6\u89e3\u65f6\u5efa\u8bae\u4f7f\u7528\u9ad8\u7eaf\u5ea6DMSO\u914d\u5236\u621010-50 mM\u6bcd\u6db2\uff0c\u5206\u88c5\u540e\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5b9e\u9a8c\u64cd\u4f5c\u9700\u5728\u751f\u7269\u5b89\u5168\u67dc\u4e2d\u8fdb\u884c\uff0c\u5e76\u4f69\u6234\u9632\u62a4\u88c5\u5907\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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