1-[8-(3-\u7532\u57fa\u82ef\u57fa)-1,4-\u4e8c\u5641\u87ba[4.5]\u7678\u70f7-8-\u57fa]\u7532\u80fa\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u672c\u4ea7\u54c1\u4e3a\u9ad8\u7eaf\u5ea6\u6709\u673a\u5316\u5408\u7269\uff0c\u5316\u5b66\u540d\u79f0\u4e3a1-[8-(3-Methylphenyl)-1,4-dioxaspiro[4.5]dec-8-yl]methanamine\uff0cCAS\u53f7887978-87-2\u3002\u5176\u5206\u5b50\u5f0f\u4e3aC16H23NO2\uff0c\u5206\u5b50\u91cf261.359\uff0c\u7eaf\u5ea6\u7ecfHPLC\u9a8c\u8bc1\u226596%\u3002\u8be5\u5316\u5408\u7269\u5177\u6709\u72ec\u7279\u7684\u87ba\u73af\u7ed3\u6784\uff0c\u542b\u82ef\u7532\u57fa\u80fa\u57fa\u56e2\u53ca\u4e8c\u6c27\u6742\u73af\u5df1\u70f7\u7247\u6bb5\uff0c\u8868\u73b0\u4e3a\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7ed3\u6676\u7c89\u672b\uff0c\u6613\u6eb6\u4e8e\u6709\u673a\u6eb6\u5242\u5982DMSO\u548c\u7532\u9187\uff0c\u5728\u9178\u6027\u6761\u4ef6\u4e0b\u53ef\u5f62\u6210\u7a33\u5b9a\u76d0\u7c7b\u3002 <\/p>\n
2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4f5c\u4e3a\u87ba\u73af\u7c7b\u884d\u751f\u7269\uff0c\u8be5\u5206\u5b50\u56e0\u5176\u521a\u6027\u7ed3\u6784\u548c\u80fa\u57fa\u6d3b\u6027\u4f4d\u70b9\uff0c\u5728\u836f\u7269\u5316\u5b66\u4e2d\u5e38\u7528\u4e8e\u6784\u5efa\u795e\u7ecf\u8c03\u8282\u5242\u6216GPCR\u9776\u5411\u5316\u5408\u7269\u7684\u6838\u5fc3\u9aa8\u67b6\u3002\u5176\u82ef\u7532\u57fa\u80fa\u7247\u6bb5\u53ef\u53c2\u4e0e\u6c22\u952e\u5f62\u6210\u548c\u9776\u6807\u7ed3\u5408\uff0c\u800c\u4e8c\u6c27\u6742\u87ba\u73af\u7ed3\u6784\u80fd\u589e\u5f3a\u4ee3\u8c22\u7a33\u5b9a\u6027\uff0c\u5728\u5148\u5bfc\u5316\u5408\u7269\u4f18\u5316\u4e2d\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u8be5\u4ea7\u54c1\u4e3b\u8981\u5e94\u7528\u4e8e\u533b\u836f\u7814\u53d1\u9886\u57df\uff0c\u5177\u4f53\u5305\u62ec\uff1a
\n1. \u4f5c\u4e3a\u4e2d\u95f4\u4f53\u7528\u4e8e\u5408\u6210\u7cbe\u795e\u795e\u7ecf\u7cfb\u7edf\u836f\u7269\uff08\u59825-HT\u53d7\u4f53\u8c03\u8282\u5242\uff09
\n2. \u7528\u4e8e\u6784\u5efa\u6297\u6291\u90c1\u6216\u6297\u7126\u8651\u6d3b\u6027\u5206\u5b50\u7684\u7ed3\u6784\u5355\u5143
\n3. \u5728\u5316\u5b66\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u4f5c\u4e3a\u63a2\u9488\u5206\u5b50\u7684\u4fee\u9970\u57fa\u56e2
\n4. \u6709\u673a\u5408\u6210\u4e2d\u7528\u4e8e\u624b\u6027\u50ac\u5316\u5242\u7684\u914d\u4f53\u5f00\u53d1 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u5efa\u8bae\u5728-20\u2103\u60f0\u6027\u6c14\u4f53\uff08\u5982\u6c29\u6c14\uff09\u4fdd\u62a4\u4e0b\u907f\u5149\u4fdd\u5b58\uff0c\u5f00\u5c01\u540e\u9700\u5145\u6c2e\u5bc6\u5c01\u3002\u4f7f\u7528\u524d\u9700\u6062\u590d\u81f3\u5ba4\u6e29\u5e76\u5e72\u71e5\u5904\u7406\uff0c\u63a8\u8350\u5de5\u4f5c\u6d53\u5ea6\u4e3a1-10 mM\uff08\u6eb6\u5242\u4e3aDMSO\uff09\u3002\u56e0\u80fa\u57fa\u6613\u6c27\u5316\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u5e94\u5728\u6c2e\u6c14\u73af\u5883\u4e0b\u8fdb\u884c\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecf\u8d28\u8c31\uff08MS\uff09\u3001\u6838\u78c1\uff081H NMR\uff09\u53ca\u5143\u7d20\u5206\u6790\uff08EA\uff09\u4e09\u91cd\u9a8c\u8bc1\uff0c\u7b26\u5408\u836f\u7269\u7814\u53d1\u7ea7\u6807\u51c6\u3002\u5b89\u5168\u6570\u636e\u8868\u660e\uff1a
\n1. \u6025\u6027\u6bd2\u6027\uff08LD50\u5927\u9f20\u53e3\u670d\uff09\uff1a\u5f85\u6d4b\u5b9a\uff08\u5efa\u8bae\u6309\u672a\u77e5\u6bd2\u6027\u7269\u8d28\u5904\u7406\uff09
\n2. \u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u62a4\u76ee\u955c\u3001\u9632\u5c18\u53e3\u7f69\u53ca\u4e01\u8148\u624b\u5957
\n3. \u82e5\u63a5\u89e6\u76ae\u80a4\uff0c\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d1715\u5206\u949f
\n4. \u5e9f\u5f03\u7269\u5e94\u4f5c\u4e3a\u6709\u5bb3\u5316\u5b66\u5e9f\u6599\u5904\u7f6e\uff0c\u7b26\u5408\u5f53\u5730\u73af\u4fdd\u6cd5\u89c4 <\/p>\n
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