2-Azido-2-deoxy-D-glucose\u4ea7\u54c1\u8bf4\u660e\u4e66 <\/p>\n
1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
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2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u4f5c\u4e3a\u8461\u8404\u7cd6\u7c7b\u4f3c\u7269\uff0c\u8be5\u5316\u5408\u7269\u53ef\u901a\u8fc7\u7ec6\u80de\u819c\u8461\u8404\u7cd6\u8f6c\u8fd0\u86cb\u767d\uff08GLUT\uff09\u8fdb\u5165\u7ec6\u80de\uff0c\u4f46\u65e0\u6cd5\u88ab\u5df1\u7cd6\u6fc0\u9176\u78f7\u9178\u5316\uff0c\u4ece\u800c\u7ade\u4e89\u6027\u6291\u5236\u7cd6\u9175\u89e3\u8fc7\u7a0b\u3002\u53e0\u6c2e\u57fa\u56e2\u7684\u5f15\u5165\u4f7f\u5176\u6210\u4e3a\u70b9\u51fb\u5316\u5b66\uff08Click Chemistry\uff09\u7684\u7406\u60f3\u5e95\u7269\uff0c\u80fd\u4e0e\u7094\u70c3\u7c7b\u5316\u5408\u7269\u53d1\u751f\u9ad8\u6548\u73af\u52a0\u6210\u53cd\u5e94\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5171\u4ef7\u6807\u8bb0\u548c\u63a2\u9488\u5408\u6210\u3002 <\/p>\n
3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u5728\u4ee3\u8c22\u7814\u7a76\u9886\u57df\uff0c\u7528\u4e8e\u7cd6\u4ee3\u8c22\u901a\u8def\u6291\u5236\u5b9e\u9a8c\u548c\u80bf\u7624\u7ec6\u80de\u4ee3\u8c22\u6210\u50cf\u3002\u5728\u5316\u5b66\u751f\u7269\u5b66\u4e2d\uff0c\u4f5c\u4e3a\u7cd6\u57fa\u5316\u4fee\u9970\u524d\u4f53\uff0c\u901a\u8fc7\u70b9\u51fb\u5316\u5b66\u53cd\u5e94\u5b9e\u73b0\u86cb\u767d\u8d28\u3001\u6838\u9178\u7b49\u751f\u7269\u5927\u5206\u5b50\u7684\u7279\u5f02\u6027\u6807\u8bb0\u3002\u836f\u7269\u5f00\u53d1\u4e2d\u7528\u4e8e\u7cd6\u7c7b\u884d\u751f\u7269\u5e93\u6784\u5efa\u548c\u6d3b\u6027\u5206\u5b50\u7b5b\u9009\u3002\u6b64\u5916\uff0c\u5728PET\u663e\u5f71\u5242\u5f00\u53d1\u4e2d\u5177\u6709\u6f5c\u5728\u5e94\u7528\u4ef7\u503c\u3002 <\/p>\n
4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u5efa\u8bae\u907f\u5149\u4fdd\u5b58\u4e8e-20\u2103\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u957f\u671f\u50a8\u5b58\u9700\u5145\u60f0\u6027\u6c14\u4f53\u4fdd\u62a4\u3002\u5f00\u5c01\u540e\u5efa\u8bae\u5206\u88c5\u4f7f\u7528\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u65f6\u9700\u5728\u901a\u98ce\u6a71\u4e2d\u64cd\u4f5c\uff0c\u907f\u514d\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u3002\u6eb6\u89e3\u65f6\u5efa\u8bae\u4f7f\u7528\u9884\u51b7\u7684PBS\u7f13\u51b2\u6db2\uff08pH 7.4\uff09\u6216\u65e0\u83cc\u6c34\uff0c\u914d\u5236\u6210\u5de5\u4f5c\u6db2\u540e\u5efa\u8bae\u5f53\u65e5\u4f7f\u7528\u5b8c\u6bd5\u3002 <\/p>\n
5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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