{"id":20998,"date":"2026-04-01T18:36:49","date_gmt":"2026-04-01T10:36:49","guid":{"rendered":"http:\/\/111.62.156.167\/index.php\/product\/5-%e4%b9%99%e7%82%94%e5%9f%ba%e5%b0%bf%e8%8b%b7\/"},"modified":"2026-04-01T22:35:46","modified_gmt":"2026-04-01T14:35:46","slug":"5-%e4%b9%99%e7%82%94%e5%9f%ba%e5%b0%bf%e8%8b%b7","status":"publish","type":"chemical-product","link":"https:\/\/bio-get.com\/?chemical-product=5-%e4%b9%99%e7%82%94%e5%9f%ba%e5%b0%bf%e8%8b%b7","title":{"rendered":"5-\u4e59\u7094\u57fa\u5c3f\u82f7"},"content":{"rendered":"<p>\u4ea7\u54c1\u8bf4\u660e<br \/>\n5-\u4e59\u7094\u57fa\u5c3f\u82f7\u4ea7\u54c1\u8bf4\u660e\u4e66<br \/>\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027<br \/>\n5-\u4e59\u7094\u57fa\u5c3f\u82f7\uff085-ethynyluridine\uff0cCAS\u53f769075-42-9\uff09\u662f\u4e00\u79cd\u4fee\u9970\u6838\u82f7\u7c7b\u4f3c\u7269\uff0c\u5206\u5b50\u5f0f\u4e3aC11H12N2O6\uff0c\u5206\u5b50\u91cf268.223\u3002\u5176\u7ed3\u6784\u5728\u5c3f\u82f7\u76845\u4f4d\u5f15\u5165\u4e59\u7094\u57fa\u56e2\uff0c\u8d4b\u4e88\u8be5\u5316\u5408\u7269\u72ec\u7279\u7684\u53cd\u5e94\u6d3b\u6027\u3002\u672c\u54c1\u4e3a\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7c89\u672b\uff0c\u7eaf\u5ea6>96%\uff0c\u53ef\u6eb6\u4e8eDMSO\u3001\u7532\u9187\u7b49\u6709\u673a\u6eb6\u5242\uff0c\u5fae\u6eb6\u4e8e\u6c34\u3002\u4e59\u7094\u57fa\u7684\u5f15\u5165\u4f7f\u5176\u6210\u4e3a\u70b9\u51fb\u5316\u5b66\uff08\u5982CuAAC\u53cd\u5e94\uff09\u7684\u7406\u60f3\u5e95\u7269\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6838\u9178\u6807\u8bb0\u4e0e\u529f\u80fd\u5316\u7814\u7a76\u3002<br \/>\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027<br \/>\n\u4f5c\u4e3a\u5c3f\u82f7\u7684\u884d\u751f\u7269\uff0c5-\u4e59\u7094\u57fa\u5c3f\u82f7\u53ef\u901a\u8fc7\u4ee3\u8c22\u9014\u5f84\u63ba\u5165RNA\u5206\u5b50\uff0c\u540c\u65f6\u4fdd\u7559\u4e59\u7094\u57fa\u56e2\u7684\u53cd\u5e94\u4f4d\u70b9\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u7814\u7a76RNA\u5408\u6210\u3001\u8f6c\u8fd0\u53ca\u964d\u89e3\u52a8\u6001\u8fc7\u7a0b\u7684\u5173\u952e\u5de5\u5177\u3002\u5176\u4e59\u7094\u57fa\u80fd\u4e0e\u53e0\u6c2e\u5316\u7269\u53d1\u751f\u7279\u5f02\u6027\u73af\u52a0\u6210\u53cd\u5e94\uff0c\u5b9e\u73b0\u8367\u5149\u6807\u8bb0\u6216\u751f\u7269\u7d20\u5316\uff0c\u4e3a\u6838\u9178\u53ef\u89c6\u5316\u4e0e\u5bcc\u96c6\u63d0\u4f9b\u9ad8\u6548\u624b\u6bb5\u3002\u5728\u8868\u89c2\u9057\u4f20\u5b66\u9886\u57df\uff0c\u8be5\u5316\u5408\u7269\u8fd8\u53ef\u7528\u4e8e\u68c0\u6d4b\u65b0\u751fRNA\u5408\u6210\u3002<br \/>\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014<br \/>\n5-\u4e59\u7094\u57fa\u5c3f\u82f7\u5e7f\u6cdb\u5e94\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a<br \/>\n\uff081\uff09RNA\u4ee3\u8c22\u7814\u7a76\uff1a\u901a\u8fc7\u70b9\u51fb\u5316\u5b66\u6807\u8bb0\u65b0\u751f\u8f6c\u5f55\u7ec4\uff1b<br \/>\n\uff082\uff09\u75c5\u6bd2\u5b66\uff1a\u8ffd\u8e2a\u75c5\u6bd2RNA\u590d\u5236\u8fc7\u7a0b\uff1b<br \/>\n\uff083\uff09\u836f\u7269\u5f00\u53d1\uff1a\u4f5c\u4e3a\u6838\u82f7\u7c7b\u836f\u7269\u7684\u524d\u4f53\u6216\u7ed3\u6784\u4fee\u9970\u6a21\u677f\uff1b<br \/>\n\uff084\uff09\u7eb3\u7c73\u6280\u672f\uff1a\u6784\u5efa\u529f\u80fd\u5316\u6838\u9178\u7eb3\u7c73\u6750\u6599\u3002\u5178\u578b\u5b9e\u9a8c\u5305\u62ecEU\u6807\u8bb0\u68c0\u6d4b\uff08\u4e0eAlexa Fluor\u53e0\u6c2e\u67d3\u6599\u8054\u7528\uff09\u3001RNA-pull down\u5206\u6790\u7b49\u3002<br \/>\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae<br \/>\n\u672c\u54c1\u9700\u907f\u5149\u4fdd\u5b58\u4e8e-20\u2103\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u6709\u6548\u671f24\u4e2a\u6708\u3002\u5f00\u5c01\u540e\u5efa\u8bae\u5206\u88c5\u4f7f\u7528\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\uff0c\u914d\u5236\u6eb6\u6db2\u65f6\u63a8\u8350\u4f7f\u7528\u65e0\u6838\u9178\u9176\u7684\u6c34\u6216\u7f13\u51b2\u6db2\u3002\u5de5\u4f5c\u6d53\u5ea6\u9700\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\uff0c\u5e38\u89c4\u7ec6\u80de\u6807\u8bb0\u6d53\u5ea6\u4e3a0.1-1 mM\u3002\u6ce8\u610f\uff1a\u4e59\u7094\u57fa\u5bf9\u5f3a\u6c27\u5316\u5242\u654f\u611f\uff0c\u5e94\u907f\u514d\u4e0e\u94dc\u76d0\u7b49\u50ac\u5316\u5242\u76f4\u63a5\u6df7\u5408\u50a8\u5b58\u3002<br \/>\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f<br \/>\n\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6>96%\uff0c\u8d28\u8c31\uff08MS\uff09\u53ca\u6838\u78c1\uff08NMR\uff09\u786e\u8ba4\u7ed3\u6784\u3002\u672c\u54c1\u5c5e\u4e8e\u523a\u6fc0\u6027\u5316\u5b66\u54c1\uff0c\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u624b\u5957\u53ca\u62a4\u76ee\u955c\u3002\u82e5\u63a5\u89e6\u76ae\u80a4\uff0c\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u7167\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002\u76f8\u5173\u7814\u7a76\u9700\u9075\u5b88\u751f\u7269\u5b89\u5168\u53ca\u4f26\u7406\u5ba1\u67e5\u8981\u6c42\uff0c\u5c24\u5176\u6d89\u53ca\u6d3b\u4f53\u5b9e\u9a8c\u65f6\u3002<br \/>\n\uff08\u6ce8\uff1a\u672c\u8bf4\u660e\u57fa\u4e8e\u73b0\u6709\u7814\u7a76\u6570\u636e\uff0c\u5b9e\u9645\u5e94\u7528\u524d\u8bf7\u67e5\u9605\u6700\u65b0\u6587\u732e\u5e76\u9a8c\u8bc1\u5b9e\u9a8c\u6761\u4ef6\u3002\uff09<\/p>\n","protected":false},"featured_media":6158,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[17],"class_list":["post-20998","chemical-product","type-chemical-product","status-publish","has-post-thumbnail","hentry","product_category-17"],"acf":[],"taxonomy_info":{"product_category":[{"value":17,"label":"\u4e2d\u95f4\u4f53"}]},"featured_image_src_large":["https:\/\/bio-get.com\/wp-content\/uploads\/2026\/03\/69075-42-9.jpg",464,464,false],"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/20998","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/media\/6158"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20998"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=20998"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}