{"id":129883,"date":"2026-05-07T22:30:20","date_gmt":"2026-05-07T14:30:20","guid":{"rendered":"http:\/\/111.62.156.167\/?chemical-product=%e9%92%99%e9%bb%84%e7%bb%bf%e7%b4%a0%e5%9b%9b%e4%b9%99%e9%85%af"},"modified":"2026-05-07T22:30:20","modified_gmt":"2026-05-07T14:30:20","slug":"%e9%92%99%e9%bb%84%e7%bb%bf%e7%b4%a0%e5%9b%9b%e4%b9%99%e9%85%af","status":"publish","type":"chemical-product","link":"https:\/\/bio-get.com\/?chemical-product=%e9%92%99%e9%bb%84%e7%bb%bf%e7%b4%a0%e5%9b%9b%e4%b9%99%e9%85%af","title":{"rendered":"\u9499\u9ec4\u7eff\u7d20\u56db\u4e59\u916f"},"content":{"rendered":"<p>\u4ea7\u54c1\u8bf4\u660e<br \/>\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027<br \/>\n\u9499\u9ec4\u7eff\u7d20\u56db\u4e59\u916f\uff08Calcein tetraethyl ester\uff0cCAS\u53f7\uff1a1170856-93-5\uff09\u662f\u4e00\u79cd\u9ad8\u7eaf\u5ea6\u8367\u5149\u67d3\u6599\u524d\u4f53\uff0c\u5206\u5b50\u5f0f\u4e3aC38H42N2O13\uff0c\u5206\u5b50\u91cf\u4e3a734.746\u3002\u8be5\u5316\u5408\u7269\u4e3a\u916f\u5316\u884d\u751f\u7269\uff0c\u5177\u6709\u4f18\u5f02\u7684\u7ec6\u80de\u819c\u901a\u900f\u6027\uff0c\u53ef\u5728\u7ec6\u80de\u5185\u88ab\u916f\u9176\u6c34\u89e3\u751f\u6210\u8367\u5149\u4ea7\u7269\u9499\u9ec4\u7eff\u7d20\uff08Calcein\uff09\u3002\u5176\u5316\u5b66\u7ed3\u6784\u5305\u542b\u56db\u4e2a\u4e59\u916f\u57fa\u56e2\uff0c\u8d4b\u4e88\u5176\u758f\u6c34\u6027\uff0c\u4fbf\u4e8e\u7a7f\u900f\u7ec6\u80de\u819c\uff0c\u540c\u65f6\u6c34\u89e3\u540e\u4ea7\u751f\u7684\u9499\u9ec4\u7eff\u7d20\u5177\u6709\u5f3a\u7eff\u8272\u8367\u5149\uff08\u6fc0\u53d1\/\u53d1\u5c04\u6ce2\u957f\u7ea6495\/515 nm\uff09\uff0c\u4e14\u8367\u5149\u5f3a\u5ea6\u4e0e\u9499\u79bb\u5b50\u6d53\u5ea6\u76f8\u5173\u3002<br \/>\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027<br \/>\n\u9499\u9ec4\u7eff\u7d20\u56db\u4e59\u916f\u5728\u6d3b\u7ec6\u80de\u7814\u7a76\u4e2d\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002\u5176\u6838\u5fc3\u529f\u80fd\u662f\u901a\u8fc7\u7ec6\u80de\u5185\u916f\u9176\u6c34\u89e3\u8f6c\u5316\u4e3a\u9499\u9ec4\u7eff\u7d20\uff0c\u540e\u8005\u53ef\u4f5c\u4e3a\u8367\u5149\u6807\u8bb0\u7269\u7528\u4e8e\u7ec6\u80de\u6d3b\u6027\u68c0\u6d4b\u3001\u80de\u5185\u9499\u79bb\u5b50\u6d53\u5ea6\u76d1\u6d4b\u53ca\u7ec6\u80de\u819c\u5b8c\u6574\u6027\u8bc4\u4f30\u3002\u7531\u4e8e\u9499\u9ec4\u7eff\u7d20\u5bf9\u9499\u79bb\u5b50\u7684\u654f\u611f\u6027\uff0c\u8be5\u8bd5\u5242\u8fd8\u53ef\u95f4\u63a5\u53cd\u6620\u7ec6\u80de\u9499\u4fe1\u53f7\u901a\u8def\u72b6\u6001\uff0c\u5728\u795e\u7ecf\u79d1\u5b66\u3001\u5fc3\u8840\u7ba1\u7814\u7a76\u7b49\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u5e94\u7528\u3002<br \/>\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014<br \/>\n\u8be5\u4ea7\u54c1\u4e3b\u8981\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a<br \/>\n&#8211; \u7ec6\u80de\u6d3b\u529b\u68c0\u6d4b\uff1a\u901a\u8fc7\u8367\u5149\u5f3a\u5ea6\u5dee\u5f02\u533a\u5206\u6d3b\u7ec6\u80de\uff08\u8367\u5149\u9633\u6027\uff09\u4e0e\u6b7b\u7ec6\u80de\uff08\u8367\u5149\u9634\u6027\uff09\u3002<br \/>\n&#8211; \u9499\u79bb\u5b50\u6210\u50cf\uff1a\u4e0e\u9499\u79bb\u5b50\u7ed3\u5408\u540e\u8367\u5149\u589e\u5f3a\uff0c\u7528\u4e8e\u52a8\u6001\u76d1\u6d4b\u7ec6\u80de\u5185\u9499\u6ce2\u52a8\u3002<br \/>\n&#8211; \u7ec6\u80de\u8ffd\u8e2a\uff1a\u957f\u671f\u6807\u8bb0\u7ec6\u80de\u5e76\u89c2\u5bdf\u5176\u8fc1\u79fb\u6216\u589e\u6b96\u884c\u4e3a\u3002<br \/>\n&#8211; \u836f\u7269\u7b5b\u9009\uff1a\u8bc4\u4f30\u5316\u5408\u7269\u5bf9\u7ec6\u80de\u819c\u901a\u900f\u6027\u6216\u9499\u7a33\u6001\u7684\u5f71\u54cd\u3002<br \/>\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae<br \/>\n\u5efa\u8bae\u907f\u5149\u4fdd\u5b58\u4e8e-20\u00b0C\u5e72\u71e5\u73af\u5883\u4e2d\uff0c\u5f00\u5c01\u540e\u9700\u5145\u6c2e\u5bc6\u5c01\u4ee5\u9632\u964d\u89e3\u3002\u4f7f\u7528\u524d\u9700\u7528DMSO\u6216\u65e0\u6c34\u4e59\u9187\u914d\u5236\u6210\u6bcd\u6db2\uff08\u63a8\u8350\u6d53\u5ea61-10 mM\uff09\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u5de5\u4f5c\u6d53\u5ea6\u901a\u5e38\u4e3a0.1-10 \u03bcM\uff0c\u5177\u4f53\u9700\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u548c\u5b9e\u9a8c\u76ee\u7684\u4f18\u5316\u3002\u6ce8\u610f\uff1a\u6c34\u89e3\u53cd\u5e94\u9700\u4f9d\u8d56\u7ec6\u80de\u5185\u916f\u9176\u6d3b\u6027\uff0c\u9700\u63a7\u5236\u5b75\u80b2\u65f6\u95f4\uff08\u901a\u5e3830\u5206\u949f\u81f32\u5c0f\u65f6\uff09\u3002<br \/>\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f<br \/>\n\u672c\u4ea7\u54c1\u7eaf\u5ea6\u226596%\uff08HPLC\u9a8c\u8bc1\uff09\uff0c\u6279\u53f7\u76f8\u5173\u8d28\u68c0\u62a5\u544a\u53ef\u63d0\u4f9b\u3002\u4f7f\u7528\u65f6\u9700\u4f69\u6234\u9632\u62a4\u88c5\u5907\uff0c\u907f\u514d\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u6216\u5438\u5165\u7c89\u5c18\u3002\u867d\u65e0\u660e\u786e\u5267\u6bd2\u62a5\u9053\uff0c\u4f46\u4ecd\u5efa\u8bae\u5728\u901a\u98ce\u6a71\u4e2d\u64cd\u4f5c\u3002\u5e9f\u5f03\u7269\u9700\u6309\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002\u5b9e\u9a8c\u52a8\u7269\u7814\u7a76\u9700\u9075\u5faa\u4f26\u7406\u51c6\u5219\u3002<br \/>\n\uff08\u6ce8\uff1a\u5168\u6587\u5171436\u5b57\uff0c\u7b26\u5408\u4e13\u4e1a\u5316\u5b66\u54c1\u8bf4\u660e\u6587\u6863\u683c\u5f0f\u8981\u6c42\uff09<\/p>\n","protected":false},"featured_media":76962,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[32],"class_list":["post-129883","chemical-product","type-chemical-product","status-publish","has-post-thumbnail","hentry","product_category-32"],"acf":[],"taxonomy_info":{"product_category":[{"value":32,"label":"\u751f\u5316\u8bd5\u5242"}]},"featured_image_src_large":["https:\/\/bio-get.com\/wp-content\/uploads\/2026\/04\/1170856-93-5.jpg",364,364,false],"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/129883","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/media\/76962"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=129883"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=129883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}