{"id":129868,"date":"2026-05-07T22:30:01","date_gmt":"2026-05-07T14:30:01","guid":{"rendered":"http:\/\/111.62.156.167\/?chemical-product=%e9%87%8d%e7%bb%84%e5%9e%8b%e4%ba%ae%e6%b0%a8%e9%85%b8%e8%84%b1%e6%b0%a2%e9%85%b6"},"modified":"2026-05-07T22:30:01","modified_gmt":"2026-05-07T14:30:01","slug":"%e9%87%8d%e7%bb%84%e5%9e%8b%e4%ba%ae%e6%b0%a8%e9%85%b8%e8%84%b1%e6%b0%a2%e9%85%b6","status":"publish","type":"chemical-product","link":"https:\/\/bio-get.com\/?chemical-product=%e9%87%8d%e7%bb%84%e5%9e%8b%e4%ba%ae%e6%b0%a8%e9%85%b8%e8%84%b1%e6%b0%a2%e9%85%b6","title":{"rendered":"\u91cd\u7ec4\u578b\u4eae\u6c28\u9178\u8131\u6c22\u9176"},"content":{"rendered":"<p>\u4ea7\u54c1\u8bf4\u660e<br \/>\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027<br \/>\n\u91cd\u7ec4\u578b\u4eae\u6c28\u9178\u8131\u6c22\u9176\uff08Leucine Dehydrogenase, recombinant\uff09\u662f\u4e00\u79cd\u9ad8\u7eaf\u5ea6\u9176\u5236\u5242\uff0cCAS\u53f7\u4e3a9082-71-7\uff0c\u7eaf\u5ea6\u226596%\u3002\u8be5\u9176\u901a\u8fc7\u57fa\u56e0\u91cd\u7ec4\u6280\u672f\u751f\u4ea7\uff0c\u5177\u6709\u4f18\u5f02\u7684\u50ac\u5316\u6d3b\u6027\u548c\u7a33\u5b9a\u6027\u3002\u5176\u5316\u5b66\u672c\u8d28\u4e3a\u86cb\u767d\u8d28\uff0c\u5206\u5b50\u5f0f\u548c\u5206\u5b50\u91cf\u56e0\u8868\u8fbe\u7cfb\u7edf\u548c\u7ffb\u8bd1\u540e\u4fee\u9970\u5dee\u5f02\u800c\u7565\u6709\u4e0d\u540c\uff0c\u4f46\u901a\u5e38\u8868\u73b0\u4e3a\u5355\u4e00\u4e9a\u57fa\u6216\u591a\u4e9a\u57fa\u590d\u5408\u4f53\u7ed3\u6784\u3002\u8be5\u9176\u5728\u751f\u7406\u6761\u4ef6\u4e0b\u53ef\u9006\u50ac\u5316L-\u4eae\u6c28\u9178\u7684\u6c27\u5316\u8131\u6c28\u53cd\u5e94\uff0c\u751f\u6210\u03b1-\u916e\u5f02\u5df1\u9178\u548cNADH\uff0c\u662f\u6c28\u57fa\u9178\u4ee3\u8c22\u901a\u8def\u4e2d\u7684\u5173\u952e\u9176\u4e4b\u4e00\u3002<br \/>\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027<br \/>\n\u4eae\u6c28\u9178\u8131\u6c22\u9176\u5c5e\u4e8e\u6c27\u5316\u8fd8\u539f\u9176\u5bb6\u65cf\uff08EC 1.4.1.9\uff09\uff0c\u7279\u5f02\u6027\u4f5c\u7528\u4e8e\u652f\u94fe\u6c28\u57fa\u9178\u4e2d\u7684L-\u4eae\u6c28\u9178\u3002\u5176\u529f\u80fd\u4f9d\u8d56\u4e8e\u8f85\u56e0\u5b50NAD+\u6216NADP+\uff0c\u5728\u6c2e\u4ee3\u8c22\u3001\u80fd\u91cf\u751f\u6210\u548c\u78b3\u9aa8\u67b6\u8f6c\u5316\u4e2d\u8d77\u6838\u5fc3\u4f5c\u7528\u3002\u8be5\u9176\u7684\u52a8\u529b\u5b66\u7279\u6027\u8868\u73b0\u4e3a\u9ad8\u5e95\u7269\u4eb2\u548c\u529b\uff08Km\u503c\u901a\u5e38\u5728\u5fae\u6469\u5c14\u7ea7\u522b\uff09\uff0c\u4e14\u5bf9\u7acb\u4f53\u5f02\u6784\u4f53\u5177\u6709\u4e25\u683c\u9009\u62e9\u6027\uff0c\u56e0\u6b64\u5728\u751f\u7269\u5408\u6210\u548c\u8bca\u65ad\u9886\u57df\u5177\u6709\u4e0d\u53ef\u66ff\u4ee3\u7684\u4ef7\u503c\u3002<br \/>\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014<br \/>\n\u8be5\u4ea7\u54c1\u5e7f\u6cdb\u5e94\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a<br \/>\n(1) \u4e34\u5e8a\u8bca\u65ad\uff1a\u4f5c\u4e3a\u8840\u6e05\u4eae\u6c28\u9178\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u6838\u5fc3\u7ec4\u5206\uff0c\u7528\u4e8e\u9057\u4f20\u4ee3\u8c22\u75c5\uff08\u5982\u67ab\u7cd6\u5c3f\u75c7\uff09\u7b5b\u67e5\uff1b<br \/>\n(2) \u751f\u7269\u50ac\u5316\uff1a\u5728\u4e0d\u5bf9\u79f0\u5408\u6210\u624b\u6027\u6c28\u57fa\u9178\u53ca\u03b1-\u916e\u9178\u884d\u751f\u7269\u4e2d\u4f5c\u4e3a\u7eff\u8272\u50ac\u5316\u5242\uff1b<br \/>\n(3) \u79d1\u7814\u5de5\u5177\uff1a\u7528\u4e8e\u7ebf\u7c92\u4f53\u529f\u80fd\u7814\u7a76\u3001\u6c28\u57fa\u9178\u4ee3\u8c22\u901a\u8def\u5206\u6790\u7b49\u57fa\u7840\u7814\u7a76\uff1b<br \/>\n(4) \u98df\u54c1\u5de5\u4e1a\uff1a\u76d1\u6d4b\u53d1\u9175\u8fc7\u7a0b\u4e2d\u652f\u94fe\u6c28\u57fa\u9178\u542b\u91cf\u53d8\u5316\u3002<br \/>\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae<br \/>\n\u63a8\u8350\u5728-20\u2103\u4ee5\u4e0b\u4f4e\u6e29\u5e72\u71e5\u4fdd\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u6eb6\u89e3\u540e\u7684\u9176\u6db2\u5e94\u5206\u88c5\u4fdd\u5b58\u4e8e4\u2103\uff08\u77ed\u671f\u5185\u4f7f\u7528\uff09\u6216-80\u2103\uff08\u957f\u671f\u4fdd\u5b58\uff09\uff0c\u6d3b\u6027\u53ef\u7ef4\u63016\u4e2a\u6708\u4ee5\u4e0a\u3002\u4f7f\u7528\u524d\u9700\u5e73\u8861\u81f3\u5ba4\u6e29\uff0c\u53cd\u5e94\u4f53\u7cfb\u5efa\u8baepH 8.0-9.0\uff08Tris-HCl\u7f13\u51b2\u6db2\uff09\uff0c\u6700\u9002\u6e29\u5ea637\u2103\u3002\u907f\u514d\u63a5\u89e6\u5f3a\u6c27\u5316\u5242\u6216\u86cb\u767d\u9176\u6291\u5236\u5242\u3002<br \/>\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f<br \/>\n\u672c\u4ea7\u54c1\u7ecfSDS-PAGE\u548cHPLC\u9a8c\u8bc1\u7eaf\u5ea6\uff0c\u6bcf\u6279\u6b21\u63d0\u4f9b\u6bd4\u6d3b\u6027\u68c0\u6d4b\u62a5\u544a\uff08\u226550 U\/mg\u86cb\u767d\uff09\u3002\u4f5c\u4e3a\u751f\u7269\u5236\u5242\uff0c\u9700\u6309\u2161\u7c7b\u5371\u9669\u5316\u5b66\u54c1\u7ba1\u7406\uff1a\u64cd\u4f5c\u65f6\u4f69\u6234\u9632\u62a4\u624b\u5957\/\u773c\u955c\uff0c\u907f\u514d\u5438\u5165\u7c89\u5c18\u6216\u63a5\u89e6\u9ecf\u819c\u3002\u5e9f\u5f03\u7269\u9700\u7ecf121\u2103\u9ad8\u538b\u706d\u83cc\u5904\u7406\u3002\u6025\u6027\u6bd2\u6027\u6570\u636e\uff08\u5927\u9f20\u53e3\u670dLD50\uff1e2000 mg\/kg\uff09\uff0c\u4f46\u8fc7\u654f\u4f53\u8d28\u8005\u4ecd\u5b58\u5728\u4e2a\u4f53\u53cd\u5e94\u98ce\u9669\u3002<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[32],"class_list":["post-129868","chemical-product","type-chemical-product","status-publish","hentry","product_category-32"],"acf":[],"taxonomy_info":{"product_category":[{"value":32,"label":"\u751f\u5316\u8bd5\u5242"}]},"featured_image_src_large":false,"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/129868","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=129868"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=129868"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}