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\u4ea7\u54c1\u8bf4\u660e
\n\u9178\u6027\u9ed1 1\uff08Amido Black 10B\uff09\u4ea7\u54c1\u8bf4\u660e
\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u9178\u6027\u9ed1 1\uff0c\u53c8\u79f0Amido Black 10B\uff0c\u662f\u4e00\u79cd\u5076\u6c2e\u7c7b\u9178\u6027\u67d3\u6599\uff0cCAS\u53f7\u4e3a1064-48-8\uff0c\u5206\u5b50\u5f0f\u4e3aC22H14N6Na2O9S2\uff0c\u5206\u5b50\u91cf\u4e3a616.491\u3002\u672c\u54c1\u4e3a\u6df1\u68d5\u8272\u81f3\u9ed1\u8272\u7c89\u672b\uff0c\u7eaf\u5ea6\u226596%\uff0c\u6613\u6eb6\u4e8e\u6c34\uff0c\u6c34\u6eb6\u6db2\u5448\u84dd\u9ed1\u8272\uff0c\u5fae\u6eb6\u4e8e\u4e59\u9187\uff0c\u4e0d\u6eb6\u4e8e\u975e\u6781\u6027\u6709\u673a\u6eb6\u5242\u3002\u5176\u5316\u5b66\u7ed3\u6784\u4e2d\u542b\u6709\u78fa\u9178\u57fa\u56e2\uff0c\u8d4b\u4e88\u5176\u826f\u597d\u7684\u6c34\u6eb6\u6027\u548c\u4e0e\u86cb\u767d\u8d28\u7ed3\u5408\u7684\u80fd\u529b\u3002
\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u9178\u6027\u9ed1 1\u5728\u751f\u7269\u5316\u5b66\u9886\u57df\u4e3b\u8981\u7528\u4e8e\u86cb\u767d\u8d28\u67d3\u8272\uff0c\u7279\u522b\u662f\u7535\u6cf3\u540e\u86cb\u767d\u8d28\u6761\u5e26\u7684\u663e\u8272\u3002\u5176\u901a\u8fc7\u4e0e\u86cb\u767d\u8d28\u7684\u6c28\u57fa\u548c\u7fa7\u57fa\u7ed3\u5408\u5f62\u6210\u590d\u5408\u7269\uff0c\u4ea7\u751f\u6df1\u8272\u6c89\u6dc0\uff0c\u4ece\u800c\u5b9e\u73b0\u5bf9\u86cb\u767d\u8d28\u7684\u9ad8\u7075\u654f\u5ea6\u68c0\u6d4b\u3002\u8be5\u67d3\u6599\u5728\u4f4e\u6d53\u5ea6\u4e0b\u5373\u53ef\u663e\u8272\uff0c\u4e14\u80cc\u666f\u67d3\u8272\u6613\u4e8e\u8131\u8272\uff0c\u662f\u86cb\u767d\u8d28\u5206\u6790\u4e2d\u7684\u91cd\u8981\u5de5\u5177\u3002
\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u9178\u6027\u9ed1 1\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u547d\u79d1\u5b66\u7814\u7a76\u548c\u4e34\u5e8a\u8bca\u65ad\u9886\u57df\u3002\u5177\u4f53\u7528\u9014\u5305\u62ec\uff1a
\n– SDS-PAGE\u3001Western Blot\u7b49\u7535\u6cf3\u6280\u672f\u7684\u86cb\u767d\u8d28\u67d3\u8272\uff1b
\n– \u7ec4\u7ec7\u5207\u7247\u548c\u7ec6\u80de\u6d82\u7247\u7684\u67d3\u8272\uff0c\u7528\u4e8e\u75c5\u7406\u5b66\u5206\u6790\uff1b
\n– \u7eba\u7ec7\u54c1\u548c\u76ae\u9769\u5de5\u4e1a\u7684\u67d3\u8272\u5242\uff1b
\n– \u58a8\u6c34\u548c\u5176\u4ed6\u5de5\u4e1a\u67d3\u6599\u7684\u6210\u5206\u3002
\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u54c1\u5e94\u5bc6\u5c01\u4fdd\u5b58\u4e8e\u5e72\u71e5\u3001\u9634\u51c9\u5904\uff0c\u907f\u514d\u9633\u5149\u76f4\u5c04\u548c\u9ad8\u6e29\uff0c\u5efa\u8bae\u50a8\u5b58\u6e29\u5ea6\u4e3a2-8\u2103\u3002\u4f7f\u7528\u524d\u9700\u5145\u5206\u6eb6\u89e3\u4e8e\u6c34\u6216\u7f13\u51b2\u6db2\uff0c\u63a8\u8350\u4f7f\u7528\u6d53\u5ea6\u4e3a0.1%-0.5%\u3002\u67d3\u8272\u540e\u53ef\u7528\u8131\u8272\u6db2\uff08\u5982\u7532\u9187-\u4e59\u9178\u6eb6\u6db2\uff09\u53bb\u9664\u80cc\u666f\u67d3\u8272\u3002\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u9632\u62a4\u624b\u5957\u548c\u773c\u955c\uff0c\u907f\u514d\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u548c\u773c\u775b\u3002
\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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