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\n\u6838\u9178\u9176\u4ea7\u54c1\u8bf4\u660e
\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u6838\u9178\u9176\uff08Nuclease\uff0cCAS\u53f7\uff1a60675-83-4\uff09\u662f\u4e00\u7c7b\u80fd\u591f\u50ac\u5316\u6838\u9178\uff08DNA\u6216RNA\uff09\u6c34\u89e3\u7684\u9176\uff0c\u5176\u5316\u5b66\u672c\u8d28\u4e3a\u86cb\u767d\u8d28\u3002\u672c\u4ea7\u54c1\u4e3a\u9ad8\u7eaf\u5ea6\u5236\u5242\uff0c\u7eaf\u5ea6\u226596%\uff0c\u5177\u6709\u9ad8\u6548\u7684\u6838\u9178\u964d\u89e3\u6d3b\u6027\u3002\u6838\u9178\u9176\u901a\u8fc7\u5207\u5272\u78f7\u9178\u4e8c\u916f\u952e\uff0c\u5c06\u6838\u9178\u5206\u89e3\u4e3a\u5be1\u6838\u82f7\u9178\u6216\u5355\u6838\u82f7\u9178\uff0c\u5176\u6d3b\u6027\u53d7pH\u3001\u6e29\u5ea6\u53ca\u91d1\u5c5e\u79bb\u5b50\uff08\u5982Mg\u00b2\u207a\u6216Ca\u00b2\u207a\uff09\u8c03\u63a7\u3002
\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\n\u6838\u9178\u9176\u5728\u751f\u7269\u4f53\u5185\u53c2\u4e0e\u591a\u79cd\u5173\u952e\u751f\u7406\u8fc7\u7a0b\uff0c\u5305\u62ecDNA\u4fee\u590d\u3001RNA\u4ee3\u8c22\u3001\u9632\u5fa1\u5916\u6e90\u6838\u9178\u5165\u4fb5\u7b49\u3002\u6839\u636e\u5e95\u7269\u7279\u5f02\u6027\uff0c\u53ef\u5206\u4e3aDNA\u9176\uff08DNase\uff09\u548cRNA\u9176\uff08RNase\uff09\uff1b\u6839\u636e\u4f5c\u7528\u65b9\u5f0f\uff0c\u53ef\u5206\u4e3a\u5185\u5207\u9176\u548c\u5916\u5207\u9176\u3002\u6838\u9178\u9176\u662f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u7684\u91cd\u8981\u5de5\u5177\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6838\u9178\u63d0\u53d6\u3001\u6837\u672c\u53bb\u6c61\u67d3\u53ca\u57fa\u56e0\u7f16\u8f91\u7b49\u5b9e\u9a8c\u3002
\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\n\u6838\u9178\u9176\u7684\u4e3b\u8981\u5e94\u7528\u5305\u62ec\uff1a
\n– \u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\uff1a\u53bb\u9664\u6837\u672c\u4e2d\u7684\u6838\u9178\u6c61\u67d3\uff0c\u5982RNA\u63d0\u53d6\u65f6\u6d88\u9664\u57fa\u56e0\u7ec4DNA\u5e72\u6270\u3002
\n– \u5236\u836f\u5de5\u4e1a\uff1a\u7528\u4e8e\u75ab\u82d7\u751f\u4ea7\u8fc7\u7a0b\u4e2d\u5bbf\u4e3b\u6838\u9178\u7684\u6e05\u9664\uff0c\u786e\u4fdd\u4ea7\u54c1\u5b89\u5168\u6027\u3002
\n– \u98df\u54c1\u68c0\u6d4b\uff1a\u964d\u89e3\u98df\u54c1\u4e2d\u7684\u6838\u9178\uff0c\u8f85\u52a9\u75c5\u539f\u4f53\u68c0\u6d4b\u6216\u6210\u5206\u5206\u6790\u3002
\n– \u4e34\u5e8a\u8bca\u65ad\uff1a\u4f5c\u4e3a\u8bd5\u5242\u7ec4\u5206\uff0c\u7528\u4e8e\u6838\u9178\u6269\u589e\u524d\u7684\u6837\u672c\u5904\u7406\u3002
\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\n\u672c\u4ea7\u54c1\u9700\u4fdd\u5b58\u4e8e-20\u2103\u5e72\u71e5\u73af\u5883\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u4f7f\u7528\u65f6\u5efa\u8bae\u5728\u51b0\u4e0a\u64cd\u4f5c\uff0c\u6eb6\u89e3\u540e\u5206\u88c5\u4fdd\u5b58\u4ee5\u7ef4\u6301\u6d3b\u6027\u3002\u53cd\u5e94\u6761\u4ef6\u901a\u5e38\u4e3a\u4e2d\u6027\u81f3\u5f31\u78b1\u6027\uff08pH 7.0-8.0\uff09\uff0c\u5e76\u9700\u6dfb\u52a0\u4e8c\u4ef7\u9633\u79bb\u5b50\uff08\u59821-10 mM MgCl\u2082\uff09\u4ee5\u6fc0\u6d3b\u9176\u6d3b\u6027\u3002\u4f7f\u7528\u6d53\u5ea6\u9700\u6839\u636e\u5b9e\u9a8c\u4f53\u7cfb\u4f18\u5316\uff0c\u907f\u514d\u8fc7\u91cf\u5bfc\u81f4\u975e\u7279\u5f02\u6027\u964d\u89e3\u3002
\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
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