{"id":128425,"date":"2026-05-07T22:07:31","date_gmt":"2026-05-07T14:07:31","guid":{"rendered":"http:\/\/111.62.156.167\/?chemical-product=%e6%9c%ab%e7%ab%af%e8%84%b1%e6%b0%a7%e6%a0%b8%e8%8b%b7%e9%85%b8%e8%bd%ac%e7%a7%bb%e9%85%b6"},"modified":"2026-05-07T22:07:31","modified_gmt":"2026-05-07T14:07:31","slug":"%e6%9c%ab%e7%ab%af%e8%84%b1%e6%b0%a7%e6%a0%b8%e8%8b%b7%e9%85%b8%e8%bd%ac%e7%a7%bb%e9%85%b6","status":"publish","type":"chemical-product","link":"https:\/\/bio-get.com\/?chemical-product=%e6%9c%ab%e7%ab%af%e8%84%b1%e6%b0%a7%e6%a0%b8%e8%8b%b7%e9%85%b8%e8%bd%ac%e7%a7%bb%e9%85%b6","title":{"rendered":"\u672b\u7aef\u8131\u6c27\u6838\u82f7\u9178\u8f6c\u79fb\u9176"},"content":{"rendered":"

\u4ea7\u54c1\u8bf4\u660e
\n\u672b\u7aef\u8131\u6c27\u6838\u82f7\u9178\u8f6c\u79fb\u9176\uff08Terminal Deoxynucleotidyl Transferase\uff0cTdT\uff09\u4ea7\u54c1\u8bf4\u660e
\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027
\n\u672b\u7aef\u8131\u6c27\u6838\u82f7\u9178\u8f6c\u79fb\u9176\uff08CAS\u53f7\uff1a9027-67-2\uff09\u662f\u4e00\u79cd\u65e0\u9700\u6a21\u677f\u7684DNA\u805a\u5408\u9176\uff0c\u80fd\u591f\u50ac\u5316\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff08dNTPs\uff09\u6dfb\u52a0\u5230DNA\u5206\u5b50\u76843′-\u7f9f\u57fa\u672b\u7aef\u3002\u8be5\u9176\u6765\u6e90\u4e8e\u5c0f\u725b\u80f8\u817a\uff0c\u5177\u6709\u9ad8\u7eaf\u5ea6\uff08\u226796%\uff09\uff0c\u5206\u5b50\u91cf\u7ea6\u4e3a58 kDa\u3002\u5176\u72ec\u7279\u7684\u975e\u6a21\u677f\u4f9d\u8d56\u6027\u4f7f\u5176\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u5177\u6709\u91cd\u8981\u4ef7\u503c\u3002
\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027
\nTdT\u5728DNA\u5408\u6210\u548c\u91cd\u7ec4\u4e2d\u53d1\u6325\u5173\u952e\u4f5c\u7528\uff0c\u5c24\u5176\u5728\u514d\u75ab\u7cfb\u7edf\u7684V(D)J\u91cd\u7ec4\u8fc7\u7a0b\u4e2d\uff0c\u8d1f\u8d23\u5728T\u7ec6\u80de\u548cB\u7ec6\u80de\u53d7\u4f53\u57fa\u56e0\u7684\u7f16\u7801\u672b\u7aef\u6dfb\u52a0\u968f\u673a\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u4ece\u800c\u589e\u5f3a\u514d\u75ab\u591a\u6837\u6027\u3002\u6b64\u5916\uff0cTdT\u5e7f\u6cdb\u5e94\u7528\u4e8eDNA\u6807\u8bb0\u3001\u63a2\u9488\u5236\u5907\u548c\u57fa\u56e0\u5de5\u7a0b\u5b9e\u9a8c\uff0c\u662f\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u7684\u91cd\u8981\u5de5\u5177\u9176\u3002
\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014
\nTdT\u7684\u4e3b\u8981\u5e94\u7528\u5305\u62ec\uff1a
\n– DNA\u672b\u7aef\u6807\u8bb0\uff1a\u901a\u8fc7\u6dfb\u52a0\u653e\u5c04\u6027\u6216\u8367\u5149\u6807\u8bb0\u7684\u6838\u82f7\u9178\uff0c\u7528\u4e8e\u6838\u9178\u6742\u4ea4\u548c\u6d4b\u5e8f\u3002
\n– \u540c\u6e90\u591a\u805a\u7269\u5c3e\u6dfb\u52a0\uff1a\u5728cDNA\u514b\u9686\u548c\u8f7d\u4f53\u6784\u5efa\u4e2d\uff0c\u7528\u4e8e\u5728DNA\u7247\u6bb5\u672b\u7aef\u6dfb\u52a0\u591a\u805aA\u6216\u591a\u805aT\u5c3e\u3002
\n– \u514d\u75ab\u5b66\u7814\u7a76\uff1a\u7528\u4e8e\u5206\u6790\u6dcb\u5df4\u7ec6\u80de\u53d1\u80b2\u8fc7\u7a0b\u4e2d\u7684\u57fa\u56e0\u91cd\u6392\u3002
\n– \u5206\u5b50\u8bca\u65ad\uff1a\u5728\u7279\u5b9a\u57fa\u56e0\u68c0\u6d4b\u548c\u7a81\u53d8\u5206\u6790\u4e2d\u4f5c\u4e3a\u8f85\u52a9\u5de5\u5177\u3002
\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae
\nTdT\u5e94\u5728-20\u00b0C\u6761\u4ef6\u4e0b\u50a8\u5b58\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u4ee5\u4fdd\u6301\u9176\u6d3b\u6027\u3002\u4f7f\u7528\u65f6\u5efa\u8bae\u7f6e\u4e8e\u51b0\u4e0a\u64cd\u4f5c\uff0c\u53cd\u5e94\u7f13\u51b2\u6db2\u901a\u5e38\u5305\u542bTris-HCl\u3001\u6c2f\u5316\u94be\u548c\u9541\u79bb\u5b50\uff0cpH 8.0-8.5\u3002\u9176\u7684\u6700\u4f73\u5de5\u4f5c\u6e29\u5ea6\u4e3a37\u00b0C\uff0c\u53cd\u5e94\u65f6\u95f4\u6839\u636e\u5b9e\u9a8c\u9700\u6c42\u8c03\u6574\uff0c\u901a\u5e38\u4e3a30\u5206\u949f\u81f31\u5c0f\u65f6\u3002
\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f
\n\u672c\u4ea7\u54c1\u7ecf\u8fc7\u4e25\u683c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u5305\u62ec\u6d3b\u6027\u68c0\u6d4b\u3001\u7eaf\u5ea6\u5206\u6790\u548c\u5185\u5207\u9176\u6b8b\u7559\u6d4b\u8bd5\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002\u4f7f\u7528\u65f6\u9700\u4f69\u6234\u9632\u62a4\u88c5\u5907\uff0c\u907f\u514d\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u6216\u773c\u775b\u3002\u5982\u4e0d\u614e\u63a5\u89e6\uff0c\u8bf7\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u5e76\u5c31\u533b\u3002\u5e9f\u5f03\u7269\u5e94\u6309\u7167\u751f\u7269\u5371\u9669\u54c1\u5904\u7406\u89c4\u8303\u5904\u7f6e\u3002<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[32],"class_list":["post-128425","chemical-product","type-chemical-product","status-publish","hentry","product_category-32"],"acf":[],"taxonomy_info":{"product_category":[{"value":32,"label":"\u751f\u5316\u8bd5\u5242"}]},"featured_image_src_large":false,"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/128425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=128425"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=128425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}