{"id":128197,"date":"2026-05-07T22:03:57","date_gmt":"2026-05-07T14:03:57","guid":{"rendered":"http:\/\/111.62.156.167\/?chemical-product=%e5%af%b9%e7%a1%9d%e5%9f%ba%e8%8b%af%e5%9f%ba-%ce%b2-d-%e5%90%a1%e5%96%83%e5%8d%8a%e4%b9%b3%e7%b3%96%e8%8b%b7pnpg"},"modified":"2026-05-07T22:03:57","modified_gmt":"2026-05-07T14:03:57","slug":"%e5%af%b9%e7%a1%9d%e5%9f%ba%e8%8b%af%e5%9f%ba-%ce%b2-d-%e5%90%a1%e5%96%83%e5%8d%8a%e4%b9%b3%e7%b3%96%e8%8b%b7pnpg","status":"publish","type":"chemical-product","link":"https:\/\/bio-get.com\/?chemical-product=%e5%af%b9%e7%a1%9d%e5%9f%ba%e8%8b%af%e5%9f%ba-%ce%b2-d-%e5%90%a1%e5%96%83%e5%8d%8a%e4%b9%b3%e7%b3%96%e8%8b%b7pnpg","title":{"rendered":"\u5bf9\u785d\u57fa\u82ef\u57fa-\u03b2-D-\u5421\u5583\u534a\u4e73\u7cd6\u82f7(PNPG)"},"content":{"rendered":"<p>\u4ea7\u54c1\u8bf4\u660e<br \/>\n1. \u4ea7\u54c1\u6982\u8ff0\u4e0e\u5316\u5b66\u7279\u6027<br \/>\n\u5bf9\u785d\u57fa\u82ef\u57fa-\u03b2-D-\u5421\u5583\u534a\u4e73\u7cd6\u82f7\uff08PNPG\uff0cCAS\u53f7\uff1a3150-24-1\uff09\u662f\u4e00\u79cd\u4eba\u5de5\u5408\u6210\u7684\u7cd6\u82f7\u7c7b\u5316\u5408\u7269\uff0c\u5316\u5b66\u5f0f\u4e3aC12H15NO8\uff0c\u5206\u5b50\u91cf\u4e3a301.249\u3002\u8be5\u5316\u5408\u7269\u7531\u5bf9\u785d\u57fa\u82ef\u915a\u4e0e\u03b2-D-\u534a\u4e73\u7cd6\u901a\u8fc7\u7cd6\u82f7\u952e\u8fde\u63a5\u800c\u6210\uff0c\u5916\u89c2\u901a\u5e38\u4e3a\u767d\u8272\u81f3\u6de1\u9ec4\u8272\u7ed3\u6676\u6216\u7c89\u672b\uff0c\u7eaf\u5ea6\u4e0d\u4f4e\u4e8e96%\u3002PNPG\u5728\u751f\u5316\u7814\u7a76\u4e2d\u56e0\u5176\u663e\u8272\u7279\u6027\u800c\u88ab\u5e7f\u6cdb\u5e94\u7528\uff0c\u5c24\u5176\u5728\u9176\u6d3b\u6027\u68c0\u6d4b\u4e2d\u8868\u73b0\u51fa\u9ad8\u7075\u654f\u5ea6\u548c\u7a33\u5b9a\u6027\u3002<br \/>\n2. \u751f\u7269\u5316\u5b66\u529f\u80fd\u4e0e\u91cd\u8981\u6027<br \/>\nPNPG\u662f\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u7684\u7ecf\u5178\u5e95\u7269\uff0c\u5728\u9176\u50ac\u5316\u4f5c\u7528\u4e0b\u53ef\u6c34\u89e3\u751f\u6210\u5bf9\u785d\u57fa\u82ef\u915a\uff08PNP\uff09\u548c\u534a\u4e73\u7cd6\u3002\u7531\u4e8ePNP\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\u5448\u73b0\u9ec4\u8272\uff0c\u5176\u5438\u5149\u5ea6\u4e0e\u9176\u6d3b\u6027\u5448\u6b63\u76f8\u5173\uff0c\u56e0\u6b64PNPG\u88ab\u5e7f\u6cdb\u7528\u4e8e\u5b9a\u91cf\u6d4b\u5b9a\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u7684\u6d3b\u6027\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u6210\u4e3a\u5206\u5b50\u751f\u7269\u5b66\u3001\u5fae\u751f\u7269\u5b66\u548c\u4e34\u5e8a\u8bca\u65ad\u9886\u57df\u7684\u91cd\u8981\u5de5\u5177\u8bd5\u5242\u3002<br \/>\n3. \u4e3b\u8981\u5e94\u7528\u9886\u57df\u4e0e\u5177\u4f53\u7528\u9014<br \/>\nPNPG\u4e3b\u8981\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a<br \/>\n&#8211; \u9176\u52a8\u529b\u5b66\u7814\u7a76\uff1a\u4f5c\u4e3a\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u7684\u4e13\u4e00\u6027\u5e95\u7269\uff0c\u7528\u4e8e\u6d4b\u5b9a\u9176\u6d3b\u6027\u53ca\u6291\u5236\u5242\u7b5b\u9009\u3002<br \/>\n&#8211; \u57fa\u56e0\u8868\u8fbe\u5206\u6790\uff1a\u5728\u5206\u5b50\u514b\u9686\u4e2d\uff0c\u4e0e\u62a5\u544a\u57fa\u56e0\uff08\u5982lacZ\uff09\u8054\u7528\uff0c\u68c0\u6d4b\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002<br \/>\n&#8211; \u5fae\u751f\u7269\u68c0\u6d4b\uff1a\u7528\u4e8e\u9274\u5b9a\u5927\u80a0\u6746\u83cc\u7b49\u8868\u8fbe\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u7684\u5fae\u751f\u7269\u3002<br \/>\n&#8211; \u8bca\u65ad\u8bd5\u5242\u5f00\u53d1\uff1a\u4f5c\u4e3a\u4e34\u5e8a\u68c0\u6d4b\u8bd5\u5242\u76d2\u7684\u6838\u5fc3\u7ec4\u5206\uff0c\u7528\u4e8e\u9057\u4f20\u4ee3\u8c22\u75c5\u7b5b\u67e5\u3002<br \/>\n4. \u50a8\u5b58\u6761\u4ef6\u4e0e\u4f7f\u7528\u5efa\u8bae<br \/>\nPNPG\u5e94\u907f\u5149\u4fdd\u5b58\u4e8e\u5e72\u71e5\u3001\u9634\u51c9\u5904\uff0c\u63a8\u8350\u50a8\u5b58\u6e29\u5ea6\u4e3a2-8\u2103\u3002\u4f7f\u7528\u65f6\u9700\u6eb6\u89e3\u4e8e\u7f13\u51b2\u6db2\uff08\u5982PBS\u6216Tris-HCl\uff09\uff0c\u5efa\u8bae\u5de5\u4f5c\u6d53\u5ea6\u4e3a1-5 mM\u3002\u7531\u4e8ePNP\u5bf9\u5149\u654f\u611f\uff0c\u53cd\u5e94\u4f53\u7cfb\u9700\u907f\u5149\u64cd\u4f5c\uff0c\u5e76\u5728405-420 nm\u6ce2\u957f\u4e0b\u68c0\u6d4b\u5438\u5149\u5ea6\u3002<br \/>\n5. \u8d28\u91cf\u63a7\u5236\u4e0e\u5b89\u5168\u4fe1\u606f<br \/>\n\u672c\u4ea7\u54c1\u7ecfHPLC\u9a8c\u8bc1\u7eaf\u5ea6\u226596%\uff0c\u6279\u95f4\u5dee\u5f02\u63a7\u5236\u5728\u00b12%\u4ee5\u5185\u3002\u4f7f\u7528\u65f6\u9700\u4f69\u6234\u9632\u62a4\u624b\u5957\u548c\u62a4\u76ee\u955c\uff0c\u907f\u514d\u5438\u5165\u7c89\u5c18\u6216\u63a5\u89e6\u76ae\u80a4\u3002\u5982\u4e0d\u614e\u63a5\u89e6\uff0c\u5e94\u7acb\u5373\u7528\u5927\u91cf\u6e05\u6c34\u51b2\u6d17\u3002\u5e9f\u5f03\u7269\u9700\u6309\u5371\u9669\u5316\u5b66\u54c1\u89c4\u8303\u5904\u7f6e\u3002MSDS\u8d44\u6599\u53ef\u968f\u8d27\u63d0\u4f9b\uff0c\u8be6\u7ec6\u8bf4\u660e\u6bd2\u7406\u5b66\u6570\u636e\uff08LD50\u7b49\uff09\u53ca\u5e94\u6025\u5904\u7406\u63aa\u65bd\u3002<\/p>\n","protected":false},"featured_media":83552,"template":"","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_category":[32],"class_list":["post-128197","chemical-product","type-chemical-product","status-publish","has-post-thumbnail","hentry","product_category-32"],"acf":[],"taxonomy_info":{"product_category":[{"value":32,"label":"\u751f\u5316\u8bd5\u5242"}]},"featured_image_src_large":["https:\/\/bio-get.com\/wp-content\/uploads\/2026\/04\/3150-24-1.jpg",364,364,false],"author_info":[],"comment_info":"","_links":{"self":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product\/128197","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/chemical-product"}],"about":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/types\/chemical-product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=\/wp\/v2\/media\/83552"}],"wp:attachment":[{"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=128197"}],"wp:term":[{"taxonomy":"product_category","embeddable":true,"href":"https:\/\/bio-get.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_category&post=128197"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}